不同梅毒螺旋体检测方法在梅毒诊断中的应用价值  

Application value of different detection methods of treponema pallidum in the diagnosis of syphilis

作　　者：刘如平 Liu Ru-ping(Department of Blood Transfusion,Xinyang Central Hospital,Xinyang 464000,Henan,China)

机构地区：[1]信阳市中心医院输血科,河南信阳464000

出　　处：《四川生理科学杂志》2024年第10期2257-2260,共4页

摘　　要：目的:探讨在梅毒患者中应用酶联免疫吸附试验(Enzyme-linked immunosorbent assays,ELISA)、明胶颗粒凝集试验(Treponema pallidum particle assay,TPPA)及化学发光免疫分析法(Chemiluminescence immunoassay,CLIA)三种梅毒螺旋体检测方法的诊断价值。方法:选取本院2023年1月至2023年12月期间收治的110例高度疑似梅毒患者为研究对象,均予以梅毒螺旋体ELISA、TPPA、CLIA检测。以符合美国疾控中心梅毒诊断与治疗指南的综合诊断结果为金标准,分析比较三种不同梅毒螺旋体检测方法的检测结果和诊断效能;并对比采用三种不同梅毒螺旋体检测方法对不同梅毒临床分期患者的检测结果。结果:梅毒螺旋体ELISA、TPPA、CLIA三种检测方法应用于梅毒诊断,其误诊率、漏诊率以及与金标准检测结果之间的一致性均无显著差异(P>0.05);且三种梅毒螺旋体检测方法的阳性预测值、阴性预测值、敏感度、特异度之间两两比较均无显著差异(P>0.05)。梅毒螺旋体ELISA与CLIA的准确度比较,梅毒螺旋体TPPA与CLIA的准确度均无显著差异(P>0.05);但梅毒螺旋体ELISA准确度与TPPA比较显著降低(P<0.05)。三种梅毒螺旋体检测方法对不同临床分期梅毒患者的误诊率、漏诊率以及与金标准检测结果之间的一致性均无显著差异(P>0.05);但梅毒螺旋体ELISA准确度显著低于TPPA(P<0.05)。结论:梅毒螺旋体ELISA、TPPA、CLIA在梅毒诊断中均具有较高的临床应用价值,且三者之间,TPPA的准确度最高。Objective:To evaluate the diagnostic value of enzyme-linked immunosorbent assay(ELISA),gelatin particle agglutination assay(TPPA),and chemiluminescence immunoassay(CLIA)for detecting treponema pallidum in patients with syphilis.Methods:A total of 110 highly suspected syphilis patients admitted to our hospital from January 2023 to December 2023 were selected as the research subjects,and all of them were tested for syphilis spirochete ELISA,TPPA,and CLIA.The comprehensive diagnostic results that meet the guidelines for syphilis diagnosis and treatment of the Centers for Disease Control and Prevention in the United States were used as the gold standard to analyze and compare the detection results and diagnostic efficacy of three different syphilis spirochete detection methods.The detection results of three different syphilis spirochete detection methods for patients with different clinical stages of syphilis were also compared.Results:The three detection methods of treponema pallidum ELISA,TPPA,and CLIA were applied to the diagnosis of syphilis,and there were no significant differences in the misdiagnosis rate,missed diagnosis rate,and consistency with the gold standard detection results(P>0.05).Moreover,there were no significant differences in the positive predictive value,negative predictive value,sensitivity,and specificity between the three treponema pallidum detection methods(P>0.05).Compared with the accuracy of treponema pallidum ELISA and CLIA,there was no significant difference in the accuracy of treponema pallidum TPPA and CLIA(P>0.05).However,the accuracy of treponema pallidum ELISA was significantly lower than that of TPPA(P<0.05).The three treponema pallidum detection methods had no significant differences in the misdiagnosis rate,missed diagnosis rate,and consistency with the gold standard detection results for patients with different clinical stages of syphilis(P>0.05).However,the accuracy of treponema pallidum ELISA was significantly lower than that of TPPA(P<0.05).Conclusion:TPPA,ELISA,and CLIA all

关 键 词：梅毒螺旋体 梅毒 诊断价值 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]