IgM亚类抗体3A6抑制ApoE^(-/-)小鼠动脉粥样硬化斑块形成  

IgM Subclass Antibody 3A6 Inhibits Atherosclerotic Plaque Formation in ApoE^(-/-)Mice

作　　者：戴慧 史小飞 郝凯凯 陈妍妍 冯旭阳 DAI Hui;SHI Xiaofei;HAO Kaikai(Department of Clinical Medicine,Gansu Medical College,Gansu 744000,China;不详)

机构地区：[1]甘肃医学院临床医学系,平凉744000 [2]空军军医大学西京医院心血管内科,西安710032

出　　处：《医学研究杂志》2024年第9期113-116,195,共5页Journal of Medical Research

基　　金：陕西省科技计划面上项目(2022JM-520);甘肃医学院校级科研项目(GY-2022ZK022)。

摘　　要：目的探究天然属性抗氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)IgM亚类抗体3A6对ApoE^(-/-)小鼠动脉粥样硬化斑块形成的影响。方法以ApoE^(-/-)小鼠为研究对象,将小鼠(18只)随机分为3组,即对照组(腹腔注射0.9%氯化钠溶液)、5G8组(腹腔注射天然属性抗LDL的IgM亚类抗体)和3A6组(腹腔注射天然属性的抗ox-LDL的IgM亚类抗体),每组各6只。高脂高胆固醇饮食饲喂12周,观察各组小鼠体质量,检测小鼠血脂的水平;苏木精-伊红染色分析小鼠主动脉的病理变化及斑块面积。结果3组小鼠的体质量、血脂水平和ox-LDL水平比较,差异无统计学意义(P=0.087);与对照组比较,3A6组小鼠动脉粥样硬化斑块面积显著减少,差异有统计学意义(P=0.023)。结论天然IgM亚类抗体3A6能够抑制ApoE^(-/-)小鼠动脉粥样硬化斑块的形成。Objective To investigate the effect of the anti-oxidized low density lipoprotein(ox-LDL)IgM subclass antibody 3A6 on the atherosclerotic plaque formation in ApoE^(-/-)mice.Methods The ApoE^(-/-)mice were randomly divided into three groups:control group(intraperitoneal injection of normal saline),5G8group(intraperitoneal injection of nature anti-LDL IgM subclass antibody)and 3A6group(intraperitoneal injection of natural anti-ox-LDL IgM subclass antibody),with 6mice in each group.The body weight of mice and the level of serum lipid in each group were measured after 12 weeks of high-fat and high-cholesterol diet.hematoxylin-eosin staining was used to analyze the pathological changes and plaque area of the aorta in mice.Results There was no significant difference in body weight,serum lipid level and ox-LDL level among the three groups(P=0.087).Compared with the control group,the area of atherosclerotic plaque was significantly decreased in the 3A6group(P=0.023).Conclusion The natural IgM subclass antibody 3A6 can effectively inhibit the formation of atherosclerotic plaque in ApoE^(-/-)mice.

关 键 词：天然抗体 3A6 氧化低密度脂蛋白 动脉粥样硬化 ApoE^(-/-) 小鼠 

分 类 号：R541[医药卫生—心血管疾病]