基于网络药理学探讨芪榆油膏加减方促进创面修复的作用机制  

Study on the Mechanism of Modified Qiyu Ointment in romoting Wound Repair based on Network Pharmacology

作　　者：代晓菊 曹波 DAI Xiaoju;CAO Bo(Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550005;The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550001)

机构地区：[1]贵州中医药大学,贵州贵阳550005 [2]贵州中医药大学第一附属医院,贵州贵阳550001

出　　处：《内蒙古中医药》2024年第9期154-160,共7页Inner Mongolia Journal of Traditional Chinese Medicine

基　　金：苗药枳稿倒、锐定谋单体靶向NF-κB、STAT3双信号通路干预UC发生发展的机制研究,地区科学基金项目(项目批准号:82160909)。

摘　　要：目的:基于网络药理学探讨芪榆油膏加减方促进创面修复的潜在作用机制。方法:通过TCMSP数据库筛选芪榆油膏加减方中各中药组分的活性成分,并利用Swiss Target Prediction数据库预测其活性成分的潜在靶点;使用Genecards数据库检索“创面修复”(Wound Repair)的相关靶点;利用Cytoscape3.7.1软件构建“药物-成分-靶点”网络,靶点为药物-疾病交集基因;通过STRING数据库构建药物与疾病交集靶点的蛋白互作网络(PPI)以获得芪榆芪榆油膏加减方促进创面修复的关键靶点;运用DAVID数据库对关键靶点进行GO功能与KEGG信号通路富集分析,并搭建药物-靶点-通路-疾病网络。结果:筛选得到芪榆油膏加减方的活性成分76个、药物靶点216个、创面修复靶点1478个,最终得到药物-疾病交集靶点118个;将PPI网络的数据进一步筛选得到AKT1、TP53、IL6、TNF、JUN、CASP3、EGFR、PTGS2、IL1B、MMP9等35位基因作为核心靶点;GO富集分析得到生物过程404条、细胞组分29种、分子功能52种,KEGG信号通路富集分析涉及到信号通路133条。结论:芪榆油膏加减方发挥作用的活性成分可能槲皮素、山奈酚、β-谷甾醇、芒柄花素、异鼠李素,多种有效成分可能是经癌症通路、IL-17信号通路、脂质与动脉粥样硬化、糖尿病并发症中的AGE-RAGE信号通路、人类巨细胞病毒感染、内分泌抵抗、TNF信号通路、癌症中的蛋白聚糖等途径作用于AKT1、TP53、IL6、TNF、JUN、CASP3、EGFR、PTGS2、IL1B、MMP9等多个核心靶点,发挥调节IL6、TNF、PTGS2、IL1B等炎性因子表达、刺激新生毛细血管生成、诱导成纤维细胞迁移、调节细胞增殖分化等多种功能干预创面愈合各进程,促进创面修复,但结果还需进一步实验验证。Objective:To explore the potential mechanism of modified Qiyu ointment in promoting wound repair based on network pharma-cology.Methods:The active ingredients of each traditional Chinese medicine component in the modified Qiyu ointment prescription wer screened through the TCMSP database,and the potential targets of the active ingredients were predicted using the SwissTargetPrediction database;the Genecards database was used to search for"wound repair"(WoundRepair)related targets;use Cytoscape 3.7.1 sofware to build a"drug-component-target"network,with the targets being drug-disease intersection genes;build a protein interaction network(PPI)of drugdisease intersection targets through the STRING database to obtain the key targets of Qiyu ointment modified prescription to promote wound repair;Use the DAVID database to enrich and analyze GO functions and KEGG signaling pathways on key targets,and build a drug-targetchannel-disease network.Results:76 active ingredients,216 drug targets,and 1478 wound repair targets were screened out,and 118 drugdisease intersection targets were finally obtained.The PPI network data was further screened to obtain 35 genes such as AKT1,TP53,IL6,TNF,JUN,CASP3,ECFR,PTGS2,ILIB,and MMP9 as core targets;CO enrichment analysis identified 404 biological processes,29 cell components,and 52 molecular functions.KEGG signaling pathway enrichment analysis involved 133 signaling pathways.Conclusion:The active ingredients of the modified Qiyu ointment may play a role in quercetin,kaempferol,β-sitosterol,formononetin,and isorhamnetin.Various active ingredients may act on AKT1,TP53,IL6,TNF,JUN,CASP3,EGFR,PTGS2,IL1B,and Atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,human cytomegalovirus infection,endocrine resistance,TNF signaling pathway,proteoglycans in cancer and other pathways.Multiple core targets such as MMP9 play various functions such as regulating the expression of inflammatory factors such as IL6,TNF,PTGS2,and ILIB,stimulating new capillary formation,inducing fibroblas

关 键 词：芪榆油膏加减方 创面修复 网络药理学 

分 类 号：R969.4[医药卫生—药理学]