基于肝脏能量代谢途径变化探讨健脾化浊消脂方对代谢相关脂肪性肝病大鼠的干预机制  

Intervention mechanism of Jianpi Huazhuo Xiaozhi formula in rats with metabolic-related fatty liver disease based on changes in energy metabolic pathways

作　　者：常婧 程秋骆 张丽霞[1,2] 王芊沣 华鹏 黄以蓉 崔翔 CHANG Jing;CHENG Qiu-luo;ZHANG Li-xia;WANG Qian-feng;HUA Peng;HUANG Yi-rong;CUI Xiang(Shaanxi University of Traditional Chinese Medicine,Xianyang Shaanxi 712046;Ankang Traditional Chinese Medicine Hospital,Ankang Shaanxi 725099)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]安康市中医医院,陕西安康725099

出　　处：《中南药学》2024年第10期2650-2655,共6页Central South Pharmacy

基　　金：陕西省自然科学基础研究计划(No.2022JM-454);陕西省中医药管理局“青年培优项目”(No.2021-PY-01);国家中医药管理局第五批优秀中医研修人才(No.国中医药人教函2022-1号);陕西省科学技术协会青年人才托举计划项目(No.20230310);安康市科技局基金(No.AK2021-SFZC-06,No.AK2020-SF-06)。

摘　　要：目的通过肝脏能量代谢途径研究健脾化浊消脂方(JPHZXZF)防治代谢相关脂肪性肝病大鼠的作用机制。方法将36只雄性SD大鼠随机分为空白组,模型组,阳性药物组,健脾化浊消脂方高、中、低剂量组。空白组饲喂维持饲料,其余五组饲喂高脂饲料同时饮用10%果葡糖浆溶液,连续喂养12周后,各给药组小鼠给予相应药物连续干预4周。末次给药后,采用生化分析仪测定大鼠血清中三酰甘油(TG)、总胆固醇(T-CHO)、低密度脂蛋白胆固醇(LDL-C)、空腹葡萄糖(FBG)含量;试剂盒法检测大鼠肝脏中TG、T-CHO、游离脂肪酸(NEFA)、柠檬酸合成酶(CS)、肉毒碱棕榈酸转移酶-1(CPT-1)、低氧诱导因子-1α(HIF-1α)、乳酸脱氢酶(LDH)含量或活性;用油红O、HE染色观察大鼠肝脏病理变化;采用蛋白免疫印迹法检测大鼠肝脏组织中p-AMPKα、HIF-1α蛋白表达水平。结果JPHZXZF可以显著降低TG、T-CHO、LDL-C、FBG、NEFA、CS、CPT-1、HIF-1α、LDH含量或活性(P<0.05),缓解肝细胞脂质沉积,升高p-AMPKα的蛋白表达水平(P<0.05),降低HIF-1α的蛋白表达水平(P<0.05)。结论JPHZXZF可以有效改善代谢相关脂肪性肝病糖脂代谢紊乱,其作用可能与改变能量代谢途径有关。Objective To determine the mechanism of Jianpi Huazhuo Xiaozhi formula(JPHZXZF)in preventing and treating metabolism-related fatty liver diseases in rats via the hepatic energy metabolism pathway.Methods Totally 36 male SD rats were randomly divided into six groups:a blank group,a model group,a positive drug group,and JPHZXZF high-dose,medium-dose,and low-dose groups.The blank group was provided with a sustaining diet,the other five groups were given high fat diet and 10%high fructose corn syrup for 12 weeks continuously.Subsequently,each treatment group received corresponding medication for 4 weeks continuously.After the final administration,the content of triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein cholesterol(LDL-C)and fasting glucose(FBG)in the rat serum was determined by biochemical analyzer.The content or activity of TG,T-CHO,free fatty acid(NEFA),citrate synthase(CS),botulinum line palmitc transferase-1(CPT-1),hypoxia-1α(HIF-1α),and lactate dehydrogenase(LDH)in the rat liver was detected by the test kit.The liver pathological changes in the rats were observed with Oil Red O and HE staining.Western blot was used to detect the expression levels of p-AMPKαand HIF-1α.Results JPHZXZF significantly reduced the expression of TG,T-CHO,LDL-C,FBG,NEFA,CS,CPT-1,HIF-1α,and LDH(P<0.05),alleviated hepatocyte lipid deposition,increased the expression of p-AMPKαprotein(P<0.05),and reduced the expression of HIF-1αprotein(P<0.05).Conclusion JPHZXZF can effectively relieved glucose and lipid disorders in metabolism-related fatty liver diseases,whose mechanism may be related to the altered energy metabolic pathways.

关 键 词：健脾化浊消脂方 能量代谢 代谢相关脂肪性肝病 

分 类 号：R286[医药卫生—中药学] R181.3[医药卫生—中医学]