HPLC图谱研究大黄“炒炭存性”  

HPLC chromatogram of“fried charcoal survivability”of rhubarb charred product

作　　者：梅明 周进[1] 阮清锋 林世和[1] MEI Ming;ZHOU Jin;RUAN Qing-feng;LIN Shi-he(Department of Pharmacy,Wuhan No.1 Hospital,Wuhan 430030)

机构地区：[1]武汉市第一医院药学部,武汉430030

出　　处：《中南药学》2024年第10期2670-2673,共4页Central South Pharmacy

基　　金：武汉市卫健委重点项目(No.WZ19A01)。

摘　　要：目的建立大黄HPLC指纹图谱,并根据主要成分峰面积的变化,分析不同炮制时间对大黄炭饮片质量的影响。方法采用Eclipse Plus C18色谱柱(4.6 mm×200 mm,5μm)色谱柱;以甲醇-0.1%磷酸水溶液为流动相,梯度洗脱;流速为1.0 mL·min^(-1);检测波长为280 nm,柱温为35℃,建立HPLC指纹图谱;采用“中药色谱指纹相似度评价系统(2012版)”对不同产地的大黄样品图谱进行处理,并对不同炮制时间的大黄炭样品进行HPLC图谱的分析与对比。结果建立的大黄HPLC指纹图谱确定了18个共有峰,通过与对照品比对,指认了8个成分,分别为没食子酸、儿茶素、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和5-羟甲基糠醛;同时,通过HPLC色谱图的比对,发现了大黄炭生成了5个新成分,发现了大黄炭不同炮制时间,各成分的变化趋势。结论建立的大黄HPLC指纹图谱方法重现性好,稳定可行;大黄的“炒炭存性”HPLC色谱图研究,可为大黄炭饮片质量控制和评价提供参考。Objective To establish the HPLC fingerprint of rhubarb and determine the effect of processing time on the quality of rhubarb based on the variation of peak area of the main components.Methods The chromatographic column was Eclipse Plus C18(4.6 mm×200 mm,5.0μm).Gradient elution was performed with methanol-0.1%phosphoric acid.The flow rate was 1.0 mL•min^(-1).The detection wavelength was 280 nm and the column temperature was 35℃.The chromatographic fingerprints of rhubarb samples from different origins were processed based on the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2012 edition)of National Pharmacopoeia Committee.The HPLC chromatograms of rhubarb charcoal samples with different processing time were compared.Results Totally 18 common peaks were identified in the established HPLC fingerprint of rhubarb.By comparing with the reference substance,8 components were identified,namely gallic acid,catechin,aloe emodin,rhein,emodin,chrysophanol,physcion,and 5-hydroxymethylfurfural.Five new components were found,and the content of each component changed with processing time.Conclusion The established rhubarb HPLC fingerprint method is reproducible and stable,which provides reference for the quality control and evaluation of rhubarb charcoal.

关 键 词：大黄 指纹图谱 炒炭 HPLC图谱 

分 类 号：R286[医药卫生—中药学] R284.1[医药卫生—中医学]