基于网络药理学探索人参皂苷Rd抗结肠癌的关键靶点及作用机制  

Screening of Key Targets and Mechanism for Colorectal Cancer Effects of Ginsenoside Rd Based on Network Pharmacology

作　　者：李慧娟 卢静 李强[1] 陆以霞[1] Li Huijuan;Lu Jing;Li Qiang(Heilongjiang Provincial Hospital,Harbin,Heilongjiang,150036,China)

机构地区：[1]黑龙江省医院,黑龙江哈尔滨150036

出　　处：《黑龙江医学》2024年第19期2315-2318,共4页Heilongjiang Medical Journal

基　　金：黑龙江省卫生健康委科研课题项目(20210303030103)。

摘　　要：目的:通过网络药理学联合分子对接技术,探讨人参皂苷Rd抗结肠癌的分子机制。方法:在Swiss Target Prediction数据库中搜索参皂苷Rd搜索对应靶点的活性成分,从GeneCards数据库及OMIM数据库筛选结肠癌的靶点,将交集靶点进行功能富集和生物学途径分析,通过Cytoscape软件绘制蛋白质互作网络(PPI),并将排序前4的核心靶点与人参皂苷Rd的进行分子对接。结果:通过网络药理学分析发现人参皂苷Rd治疗结肠癌的关键靶点为44个;本体富集(GO)分析结果显示,包括2 406个生物过程(BP)、48个细胞成分(CC)和169个分子功能(MF);京都基因与基因组百科全书(KEGG)结果发现了91个信号通路。将排列前4的核心靶点与人参皂苷Rd进行分子对接,结果表明人参皂苷Rd对关键靶点具有有效的结合热。结论:MMP9、IL2、NR3C1、JUN、PTGS2、STAT3、PRSS1、F2RL1、CYP27B1和F2是人参皂苷Rd治疗结肠癌的关键靶点,可能通过PI3K/Akt信号通路、MAPK信号通路、Wnt信号通路等抑制结肠癌,后续可通过基础实验进一步验证。Objective:To explore the molecular mechanism of ginsenoside Rd on colorectal cancer(CRC)by network pharmacology and molecular docking.Methods:Swiss Target Prediction database was used to screen the ginsenoside Rd targets.The targets of colon cancer were screened from GeneCards database and OMIM database,and the intersection targets were analyzed for functional enrichment and biological pathways.PPI network map was drawn by Cytoscape software,and molecular docking was conducted between the top 4 core targets and ginsenoside Rd.Results:Network pharmacological analysis showed that there were 44 key targets of ginsenoside Rd in the treatment of CRC.The results of the GO analysis showed that 2046 biological processes(BP),48 cell components(CC)and 169 molecular functions(MF)were included.Molecular docking analysis of the top 4 hub genes with ginsenoside Rd demonstrated that ginsenoside Rd had effective energy for the key targets.Conclusion:MMP9,IL2,NR3C1,JUN,PTGS2,STAT3,PRSS1,F2RL1,CYP27B1 and F2 are the key targets of ginsenoside Rd in the treatment of colon cancer,which may inhibit colon cancer through PI3K/Akt signaling pathway,MAPK signaling pathway,Wnt signaling pathway,etc.It can be further verified by basic experiments.

关 键 词：人参皂苷RD 结肠癌 网络药理学 作用机制 分子对接 

分 类 号：R96[医药卫生—药理学]