南阳市儿童反复呼吸道感染与肠道微生态变化的相关性调查  

Correlation Between Recurrent Respiratory Tract Infections and Intestinal Microecology in Children in Nanyang City

作　　者：李飒 陈静[1] 李月明 Li Sa;Chen Jing;Li Yueming(The Second Department of Pediatrics,Nanyang First People’s Hospital Affiliated to Henan University,Nanyang,Henan,473000,China)

机构地区：[1]河南大学附属南阳市第一人民医院儿二科,河南南阳473000

出　　处：《黑龙江医学》2024年第20期2514-2516,2520,共4页Heilongjiang Medical Journal

摘　　要：目的:分析南阳市儿童反复呼吸道感染(RRTI)患儿临床特征与肠道微生态状况的相关性,为RRTI患儿的预防及治疗方案制定提供参考。方法:选取2018—2021年河南大学附属南阳市第一人民医院收治的218例符合条件的RRTI患儿作为RRTI组,按照1∶1比例纳入同期同医院行健康体检的218名儿童作为传统组。收集所有纳入受试儿童的年龄、性别、喂养方式、体重、身高等信息并分组比较。明确并整理观察组患儿感染部位、1年内感染频次、淋巴细胞百分比(CD3+、CD4+、CD4+/CD8+)数据。应用16S rRNA荧光定量PCR检测所有受试儿童肠道中双歧杆菌和大肠杆菌菌落数,计算B/E值并行组间比较。比较观察组患儿中不同感染部位、不同感染频次、不同淋巴细胞百分比范围患儿双歧杆菌、大肠杆菌、双歧杆菌与大肠杆菌比值(B/E值)。以B/E值<1为肠道微生态紊乱,分析反复呼吸道感染患儿感染部位、不同感染频次、不同淋巴细胞百分比与肠道微生态异常间的相关性。结果:RRTI组患儿肠道双歧杆菌菌落数、B/E值明显低于传统组受试儿童,大肠杆菌数明显高于传统组,差异均有统计学意义(t=12.727、22.466、24.572,P<0.05);RRTI患儿下呼吸道感染、1年内感染频次与肠道微生态紊乱呈负相关(r=-0.621、-0.703);治疗后,RRTI组患儿CD3+、CD4+/CD8+高于传统组、CD4+低于传统组,差异均有统计学意义(t=5.312、4.799、4.424,P<0.05);治疗后,RRTI组患儿大肠埃希菌、肠球菌数量低于传统组、双歧杆菌数量低于传统组,差异均有统计学意义(t=5.874、6.031、8.192,P<0.05)。结论:南阳市儿童反复呼吸道感染相关指标与肠道微生态紊乱呈高度相关,临床应高度关注反复呼吸道感染患儿的肠道微生态平衡调整,以期提高反复呼吸道感染防治效率,促进儿童健康水平。Objective:To analyze the correlation between clinical features and intestinal microecological status in children with recurrent respiratory tract infection(RRRI)in Nanyang City,and to provide reference for the prevention and treatment of RRRI.Methods:218 eligible children with recurrent respiratory tract infection in Hennan Children’s Hospital were enrolled as the observation group,and 218 children who underwent physical examination in the same hospital were enrolled as the control group according to the ratio of 1∶1.Age,sex,feeding style,weight and height of all children were collected and compared.The infection site,infection frequency in a year and lymphocyte percentage(CD3+,CD4+and CD4+/CD8+)in the observation group were determined and sorted out.16S rRNA fluorescence quantitative PCR was used to detect the number of Bifidobacterium and Escherichia coli colonies in the intestinal tract of all children,and the B/E value was calculated and compared between groups.The bifidobacteria,Escherichia coli and B/E values of children with different infection sites,different infection frequencies and different lymphocyte percentage ranges were compared in the observation group.With B/E value<1,the correlation between the infection site,different infection frequency,different lymphocyte percentage and intestinal microecological abnormalities in children with recurrent respiratory tract infection was analyzed.Results:The number of intestinal bifidobacterium colonies and B/E value of children in RRTI group were significantly lower than those in traditional group,and the number of E.coli was significantly higher than that in traditional group,with statistically significant difference(t=12.727,22.466,24.572;P<0.05).The lower respiratory tract infection and the frequency of infection within 1 year were negatively correlated with intestinal microecological disturbance in children with RRTI(r=-0.621,-0.703).After treatment,CD3+and CD4+/CD8+in RRTI group were higher than those in traditional group,and CD4+were lower than thos

关 键 词：儿童反复呼吸道感染 肠道微生态平衡 双歧杆菌 大肠杆菌 免疫功能 相关性 防治对策 

分 类 号：R181.3[医药卫生—流行病学]