针艾结合疗法调控“肾-脑-天癸”系统对PDD大鼠小胶质细胞极化的影响  

Effects of Acupuncture Combined with Moxibustion on Polarization of Microglia in Rats with PDD by Regulating“Kidney-Brain-Tiangui”System

作　　者：时柳清 任路[2] 吕琴 陈海洋 鲁美 周歆 SHI Liuqing;REN Lu;LYU Qin;CHEN Haiyang;LU Mei;ZHOU Xin(Graduate School of Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;College of Acupuncture-Moxibustion and Tuina of Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China)

机构地区：[1]辽宁中医药大学研究生学院,沈阳110847 [2]辽宁中医药大学,沈阳110847 [3]辽宁中医药大学针灸推拿学院,沈阳110847

出　　处：《辽宁中医杂志》2024年第10期170-175,I0003-I0005,共9页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(82274665);辽宁省特聘教授项目(辽教发〔2015〕153号)。

摘　　要：目的观察针艾结合疗法对围绝经期抑郁症(PDD)大鼠海马区小胶质细胞极化的影响,探讨针艾结合疗法治疗PDD的潜在机制。方法将36只SPF级SD雌性大鼠随机分为空白组、模型组、西药组、电针组、艾灸组、针艾组,每组6只。除空白组外,其他组均采用慢性不可预见性温和刺激(CUMS)联合卵巢摘除术(OVX)建立PDD模型。西药组予百忧解(1.8 mg/kg)灌胃,1次/d,共21 d;电针组针刺“百会”、双侧“肾俞”、双侧“太溪”(疏密波,频率在4-20Hz内规律性变化,15 min/次,百会穴不连电针仪),1次/d,共21 d;艾灸组取背部足太阳膀胱经予温和灸,20 min/次,1次/3 d,共21 d;针艾组予以电针联合艾灸疗法,电针1次/d,艾灸1次/3 d,共21 d。通过糖水偏好、强迫游泳、旷场实验对各组大鼠进行行为学评估;HE染色、尼氏染色观察神经元排列及尼氏小体;免疫荧光法检测小胶质细胞离子钙接头蛋白分子1(IBA-1)阳性表达;ELISA法检测大鼠血清多巴胺、大鼠肿瘤坏死因子α(TNF-α)、白细胞介素-10(IL-10)水平;Western blot法检测大鼠海马区白细胞介素-1β(IL-1β)、白细胞介素-4(IL-4)水平以及诱导型一氧化氮合酶(iNOS)、精氨酸酶-1(Arg-1)表达水平。结果与空白组比较,模型组抑郁样行为明显增多(P<0.01);神经元排列紊乱稀疏,细胞形态改变;血清TNF-α含量、海马区IL-1β蛋白水平、IBA-1阳性率、iNOS表达明显增加(P<0.001,P<0.05,P<0.01,P<0.01),多巴胺、IL-10含量、IL-4蛋白水平、Arg-1表达明显降低(P<0.01,P<0.001,P<0.01,P<0.01)。与模型组比较,治疗组抑郁样行为明显减少(P<0.05);神经元排列较为规整紧密,细胞形态无明显异常;血清TNF-α含量、海马区IL-1β蛋白水平、IBA-1阳性率、iNOS表达降低(P<0.001,P<0.01,P<0.05,P<0.05),多巴胺、IL-10含量、IL-4蛋白水平、Arg-1表达升高(P<0.05,P<0.01,P<0.05,P<0.05)。结论基于“肾-脑-天癸”系统的针艾结合疗法可以在一定程度上改�Objective To explore the potential mechanism of electroacupuncture combined with moxibustion(EA+MOX)in the treatment of perimenopausal depression disorder(PDD)by observing the effect of EA+MOX on the polarization of hippocampal microglia in PDD rats.Methods Thirty-six SPF-grade SD female rats were randomly divided into blank,model,western medicine(Prozac),electroacupuncture(EA),moxibustion(MOX)and EA+MOX groups,with six rats in each group.Except the blank group,all other groups used chronic unpredictable mild stress(CUMS)combined with ovariectomize(OVX)to establish the PDD model.The rats in the western medicine group were treated with Prozac(1.8 mg/kg)by gavage once a day for 21 days.In the EA group,acupuncture was applied to“Baihui”(GV20),bilateral“Shenshu”(BL23)and bilateral“Taixi”(BL23)[disperse-dense wave,frequency changing regularly within 4-20 Hz,15 min for each time,without electroacupuncture device at“Baihui”(GV20)],once a day for 21 days.In the MOX group,mild moxibustion was applied at the urinary bladder meridian of foot-Taiyang on the back,20 min for each time,once every 3 days,for 21 days.The EA+MOX group were treated with EA combined with MOX.EA was applied once a day and moxibustion was applied once every 3 days for 21 days.The behavior of rats in each group was evaluated by sucrose preference,forced swimming and open field test.HE staining and Nissl staining were used to observe neuronal arrangement and Nissl bodies.The positive expression of ionized calcium binding adapter molecule 1(IBA-1)in microglia was detected by immunofluorescence.The serum levels of dopamine,tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)were detected by ELISA.The levels of interleukin-1β(IL-1β),interleukin-4(IL-4)and the expressions of inducible nitric oxide synthase(iNOS)and arginase-1(Arg-1)in the hippocampus were detected by Western blot.Results Compared with those in the blank group,the depression-like behaviors in the model group were significantly increased(P<0.01).The neurons were arrange

关 键 词：“肾-脑-天癸”系统 围绝经期抑郁症 小胶质细胞极化 神经元炎症 电针 艾灸 

分 类 号：R245[医药卫生—针灸推拿学]