基于IL-23/Th-17信号通路探讨运脾泻肺化痰汤对幼龄哮喘大鼠气道炎症、黏液高分泌的影响  

Effect of Yunpi Xiefei Huatan Decoction(运脾泻肺化痰汤)on Airway Inflammation and Mucus Hypersecretion in Young Asthmatic Rats Though IL-23/Th-17 Signal Pathway

作　　者：王文洲 高倩倩 陈竹 WANG Wenzhou;GAO Qianqian;CHEN Zhu(Second Clinical Medical College of Guizhou University of Chinese Medicine,Guiyang 550002,Guizhou,China;The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,Guizhou,China)

机构地区：[1]贵州中医药大学第二临床医学院,贵州贵阳550002 [2]贵州中医药大学第二附属医院,贵州贵阳550001

出　　处：《辽宁中医杂志》2024年第10期193-197,I0008,F0003,共7页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金地区科学基金资助项目(81860868);贵州省科技厅项目(﹝2020﹞1Y365)。

摘　　要：目的基于IL-23/Th-17信号通路探讨运脾泻肺化痰汤对幼龄哮喘大鼠气道炎症、黏液高分泌影响。方法将40只雌雄均等的幼龄SD大鼠,随机等分为4组:正常组(Normal group)、模型组(Model group)、运脾泻肺化痰汤组(TCM group)和布地奈德组(Bbudesonide group),每组10只。采用分段致敏及激发,实验第1、8天Medel group、TCM group及Bbudesonide group利用腹腔注射卵清白蛋白联合氢氧化铝凝胶致敏;实验第15天开始吸入1%卵清蛋白雾化激发,隔天1次,共7次。第16天开始造模并同时给药,正常组、模型组、运脾泻肺化痰汤灌胃量均为2 mL,前两组予以0.9%氯化钠,后者予运脾泻肺化痰汤。布地奈德组予0.01%布地奈德雾化吸入20 min,连续4周。末次激发后24 h内进行模型鉴定及取材。HE、PAS染色观察肺组织病理改变,ELISA检测肺泡灌洗液及血清中白细胞介素-17、白细胞介素-23炎症水平,WB检测肺组织MUC5AC蛋白含量,qPCR检测肺组织中的IL-17、IL-23及MUC5AC mRNA相对表达量。IHC测量肺部IL-17、IL-23水平。对BALF中白细胞总数、嗜酸性粒细胞及中性粒细胞进行计数。结果与正常组比较,模型组血清及肺泡灌洗IL-17、IL-23水平升高(P<0.001,P<0.0001),IL-17、IL-23免疫组化半定量及转录水平增加,MUC5A基因及蛋白表达升高(P<0.05),运脾泻肺化痰汤组、布地奈德组较模型组下降(P<0.05),模型组BALF中白细胞、嗜酸性粒细胞、中性粒细胞计数均升高(P<0.001,P<0.0001),各治疗组中性粒细胞、嗜酸性粒细胞差异无统计学意义(P>0.05)。结论运脾泻肺化痰汤降低血清及肺泡灌洗中IL-23、IL-17水平,减少炎细胞迁移、气道炎性浸润及杯状细胞增生从而抑制MUC5AC过表达,减轻气道黏液高分泌及气道炎症。Objective To explore the effect of YunpiXiefeiHuatan Decoction on airway inflammation and mucus hypersecretion in young asthmatic rats based on the IL-23/Th-17 signaling pathway.Methods Forty female young SD rats of equal weight were randomly divided into 4 groups:Normal group,Model group,YunpiXiefeiHuatan Decoction group(TCM group),and Budesonide group,with 10 rats in each group.Segmented sensitization and challenge were employed.On the 1st and 8th days of the experiment,the Model group,TCM group,and Budesonide group were sensitized via intraperitoneal injection of ovalbumin combined with aluminum hydroxide gel.Inhalation of 1%ovalbumin atomization challenge was initiated on the 15th day of the experiment,once every other day,for a total of 7 times.Modeling and administration began on the 16th day.The Normal group and Model group were gavaged with 2 mL of 0.9%sodium chloride,the TCM group was gavaged with 2 mL of YunpiXiefeiHuatan Decoction,and the Budesonide group was given 0.01%budesonide inhalation for 20 minutes,continuously for 4 weeks.Model identification and sample collection were carried out within 24 hours after the last challenge.HE and PAS staining were utilized to observe the pathological changes of lung tissue.ELISA was used to detect the inflammatory levels of interleukin-17 and interleukin-23 in bronchoalveolar lavage fluid and serum.WB was used to detect the protein content of MUC5AC in lung tissue.qPCR was used to detect the relative expression levels of IL-17,IL-23,and MUC5AC mRNA in lung tissue.IHC was used to measure the levels of IL-17 and IL-23 in the lungs.The total number of white blood cells,eosinophils,and neutrophils in BALF was counted.Results Compared with the Normal group,the levels of IL-17 and IL-23 in the serum and bronchoalveolar lavage of the Model group increased(P<0.001,P<0.0001),the semi-quantitative and transcriptional levels of IL-17 and IL-23 in immunohistochemistry rose,and the expression of the MUC5A gene and protein increased(P<0.05).The YunpiXiefeiHuatan Decoction gro

关 键 词：支气管哮喘 运脾泻肺化痰汤 白细胞介素-17 白细胞介素-23 黏蛋白5AC 

分 类 号：R285.5[医药卫生—中药学]