Lnc-H2AFV-1促进头颈鳞癌细胞的增殖、迁移、侵袭及顺铂耐药性的研究  

Research of Lnc-H2AFV-1 promoting proliferation,migration,invasion and cisplatin resistance of head and neck squamous cell cancer cells

作　　者：王栋 孙馨辰 王君[3] WANG Dong;SUN Xin-chen;WANG Jun(School of Medicine,Nantong University,Nantong 226000,Jiangsu Province,China;Department of Laboratory,Taizhou People's Hospital,Taizhou 225300,Jiangsu Province,China;Emergency,Taizhou People's Hospital,Taizhou 225300,Jiangsu Province,China)

机构地区：[1]南通大学医学院,江苏南通226000 [2]泰州市人民医院检验科,江苏泰州225300 [3]泰州市人民医院急诊科,江苏泰州225300

出　　处：《中国临床药理学杂志》2024年第19期2831-2836,共6页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探究长链非编码RNA(LncRNA)Lnc-H2AFV-1通过调控SEC11同源物A(SEC11A)表达对头颈鳞癌(HNSCC)细胞的增殖、迁移、侵袭及顺铂耐药性的影响。方法CAL-27细胞分为NC组(转染si-NC)、si-H2AFV-1组(敲低Lnc-H2AFV-1)、共转染组(敲低Lnc-H2AFV-1+过表达SEC11A)。用实时荧光定量聚合酶链反应检测细胞中SEC11A mRNA相对表达水平,用蛋白质印迹法检测各组SEC11A蛋白相对表达水平,用5-乙炔基-2′脱氧尿苷实验法检测各组细胞增殖率,用Transwell实验法评估各组细胞的侵袭能力,用细胞划痕实验检测各组细胞的迁移能力,用细胞计数试剂盒8实验法检测各个细胞分组对顺铂耐药性。结果NC组和si-H2AFV-1组的SEC11A mRNA相对表达水平分别为1.00±0.10和0.43±0.06,SEC11A蛋白相对表达水平分别为1.00±0.13和0.36±0.04,在统计学上差异均有统计学意义(均P<0.05)。NC组、si-H2AFV-1组和共转染组的细胞增殖率分别为(43.73±3.68)%、(10.82±1.75)%和(51.66±4.91)%,细胞迁移率分别为(43.51±4.63)%、(13.76±2.39)%和(54.29±5.91)%,穿过小室细胞数分别为(189.65±18.27)、(65.42±5.48)和(196.44±19.86)个,半抑制浓度(IC_(50))分别为12.61、5.37和14.76μg·mL^(-1),si-H2AFV-1组的上述指标与NC组和共转染组比较,在统计学上差异均有统计学意义(均P<0.05)。结论Lnc-H2AFV-1可以通过调控SEC11A表达来促进HNSCC细胞的增殖、迁移、侵袭及顺铂耐药性。Objective To investigate the effect of long non-coding RNA(LncRNA)Lnc-H2AFV-1 on the proliferation,migration,invasion and cisplatin resistance of head and neck squamous cell carcinoma(HNSCC)cells by regulating the expression of SEC11homolog A(SEC11A).Methods CAL-27 cells were divided into NC group(transfected with si-NC),si-H2AFV-1 group(knocked down Lnc-H2AFV-1),cotransfection group(knocked down Lnc-H2AFV-1+overexpressed SEC11A).The relative expression level of SEC11A mRNA in each cell line and cell group was detected by real-time fluorescence quantitative polymerase chain reaction.The relative expression level of SEC11A protein in each group was detected by Western blotting.The proliferation rate of each group was detected by 5-ethynyl-2'-deoxyuridine assay.The invasion ability of each group was evaluated by Transwell chamber assay.The migration ability of each group was detected by cell scratch assay.The resistance of each cell group to cisplatin was detected by cell counting kit-8 assay.Results The relative expression levels of SEC11A mRNA in the NC group and the si-H2AFV-1 group were1.00±0.10 and 0.43±0.06,respectively;the relative expression levels of SEC11A protein were 1.00±0.13 and0.36±0.04,respectively.The differences between the two groups were statistically significant(all P<0.05).The cell proliferation rates of NC group,si-H2AFV-1 group and cotransfection group were(43.73±3.68)%,(10.82±1.75)%and(51.66±4.91)%,respectively;the cell migration ratios were(43.51±4.63)%,(13.76±2.39)%and(54.29±5.91)%,respectively;the number of cells passing through the chamber was(189.65±18.27),(65.42±5.48)and(196.44±19.86),respectively.The half maximal inhibitory concentration(IC_(50))were 12.61,5.37 and 14.76μg·mL^(-1),respectively.The above indexes in the si-H2AFV-1 group were statistically significant compared with the NC group and the cotransfection group(all P<0.05).Conclusion Lnc-H2AFV-1 can promote the proliferation,migration,invasion and cisplatin resistance of HNSCC cells by regulating SEC11A expres

关 键 词：长链非编码RNA 头颈鳞癌 SEC11同源物A 舌鳞癌 增殖 迁移 顺铂耐药性 

分 类 号：R97[医药卫生—药品]