芍药苷调控血管内皮生长因子对小鼠宫颈癌组织病理变化的影响  

Effects of paeoniflorin regulating vascular endothelial growth factor on the pathological changes of cervical cancer in mice

作　　者：程亚[1] 高伟[1] 王钟辉[1] CHENG Ya;GAO Wei;WANG Zhong-hui(Department of Pathology,Shangqiu Medical College,Shangqiu 476100,Henan Province,China)

机构地区：[1]商丘医学高等专科学校病理教研室,河南商丘476100

出　　处：《中国临床药理学杂志》2024年第19期2875-2879,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨芍药苷通过靶向调控血管内皮生长因子(VEGF)对宫颈癌移植瘤小鼠肿瘤组织病理变化的影响。方法将小鼠随机分为模型组(构建宫颈癌移植瘤小鼠模型)、芍药苷组(建模后给予100 mg·kg^(-1)芍药苷)、Vector组(建模后给予100 mg·kg^(-1)芍药苷+瘤体注射Vector质粒)、VEGF组(建模后给予100 mg·kg^(-1)芍药苷+瘤体注射过表达VEGF质粒),建模成功后每隔7 d测量肿瘤体积,用免疫组化法检测肿瘤微血管密度(MVD),用蛋白质印迹法检测肿瘤组织增殖、凋亡及转移相关蛋白的表达水平,用原位末端转移酶标记法检测肿瘤组织的细胞凋亡率。结果模型组、芍药苷组、Vector组和VEGF组建模成功后第28天的肿瘤体积分别为(1025.50±81.38)、(722.89±49.67)、(720.36±64.01)和(946.79±89.77)mm^(3),CD34-MVD水平分别为118.92±6.93、71.67±7.45、72.14±5.55和86.74±8.86,VEGF蛋白相对表达水平分别为1.08±0.09、0.56±0.09、0.54±0.06和0.88±0.13,细胞周期蛋白D1(Cyclin D1)蛋白相对表达水平分别为0.93±0.14、0.44±0.03、0.46±0.07和0.81±0.09,基质金属蛋白9(MMP-9)蛋白相对表达水平分别为1.17±0.12、0.62±0.07、0.64±0.08和0.90±0.07,细胞凋亡率分别为(4.13±0.47)%、(22.16±2.84)%、(21.24±3.42)和(8.60±0.96)%;芍药苷组的上述指标与VEGF组和模型组比较,在统计学上差异均有统计学意义(均P<0.01)。结论芍药苷可能通过调节VEGF的表达来抑制血管生成,改变肿瘤组织病理学变化,抑制肿瘤生长,从而缓解小鼠宫颈癌进程。Objective To investigate the effect of paeoniflorin on the pathological changes of tumor tissue in mice transplanted with cervical cancer through targeted regulation of vascular endothelial growth factor(VEGF).Methods The successfully constructed mice were randomly divided into model group(construct a mouse model of cervical cancer transplantation tumor),paeoniflorin group(100 mg·kg^(-1)paeoniflorin treatment was given after modeling),Vector group(100 mg·kg^(-1)paeoniflorin+tumor injection of Vector plasmid after modeling),VEGF group(after modeling,100 mg·kg^(-1)paeoniflorin+tumor injection of overexpressed VEGF plasmid).After successful modeling,the tumor volume was measured every 7 days.Immunohistochemical method was used to detect tumor micro vascular density(MVD).Western blot assay was used to detect proteins related to tumor proliferation,apoptosis and metastasis.The apoptosis rate of tumor tissue was detected by TdT mediated dUDP nick end labeling(Tunel)assay.Results The 28 d tumor volume of model group,paeoniflorin group,Vector group and VEGF group were(1025.50±81.38),(722.89±49.67),(720.36±64.01)and(946.79±89.77)mm^(3),respectively;VEGF protein expression levels were 1.08±0.09,0.56±0.09,0.54±0.06 and 0.88±0.13,respectively;CD34-MVD levels were 118.92±6.93,71.67±7.45,72.14±5.55 and 86.74±8.86;the relative expression levels of Cyclin D1 were 0.93±0.14,0.44±0.03,0.46±0.07 and 0.81±0.09,respectively;the relative expression levels of matrix metalloprotein-9(MMP-9)were1.17±0.12,0.62±0.07,0.64±0.08 and 0.90±0.07,respectively;the apoptosis rates were(4.13±0.47)%,(22.16±2.84)%,(21.24±3.42)%and(8.60±0.96)%,respectively.The above indexes of paeoniflorin group were significantly different from those of model group(all P<0.01);the above indexes of VEGF group were significantly different from those of paeoniflorin group(all P<0.01).Conclusion Paeoniflorin may inhibit angiogenesis,change tumor histopathological changes,inhibit tumor growth and alleviate the progression of cervical cancer in mi

关 键 词：芍药苷 宫颈癌 血管内皮生长因子 组织病理 抗血管生成 

分 类 号：R28[医药卫生—中药学]