神农架大九湖泥炭孔隙水中微生物的碳代谢特征  

Characteristics of Microbial Carbon Utilization in Peat Pore Water in the Dajiuhu Peatland,Shennongjia

作　　者：田文 王红梅[3] 向兴 王锐诚 黄咸雨[2] Tian Wen;Wang Hongmei;Xiang Xing;Wang Ruicheng;Huang Xianyu(College of Resource and Environment,Anhui Science and Technology University,Chuzhou 233100,China;Hubei Key Laboratory of Critical Zone Evolution,China University of Geosciences,Wuhan 430078,China;State Key Laboratory of Biogeology and Environmental Geology,China University of Geosciences,Wuhan 430078,China;College of Life Science,Shangrao Normal University,Shangrao 334001,China)

机构地区：[1]安徽科技学院资源与环境学院,安徽滁州233100 [2]中国地质大学流域关键带演化湖北省重点实验室,湖北武汉430078 [3]中国地质大学生物地质与环境地质国家重点实验室,湖北武汉430078 [4]上饶师范学院生命科学学院,江西上饶334001

出　　处：《地球科学》2024年第9期3241-3251,共11页Earth Science

基　　金：国家自然科学基金项目(No.41572325);流域关键带演化湖北省重点实验室开放基金项目(No.CEZ2022F02).

摘　　要：为研究水位波动下泥炭地微生物碳代谢特征,利用Biolog-Eco微平板技术对神农架大九湖不同水位下泥炭孔隙水中微生物的碳代谢图谱进行测定.结果表明,不同水位下微生物的碳代谢活性和多样性差异显著,均表现为中水位>低水位>高水位.酯类(丙酮酸甲酯、吐温40、吐温80、D-半乳糖酸γ内酯),氨基酸(L-精氨酸、L-天冬酰胺酸、L-苯基丙氨酸、L-丝氨酸和甘氨酰-L-谷氨酸),胺类(苯乙基胺、腐胺和N-乙酰基-D-葡萄胺)是引起微生物碳代谢差异的主要贡献者.冗余度分析显示,电导率(F=3.2,P=0.018)和氧化还原电位(F=2.6,P=0.044)显著影响微生物的碳代谢,其变化与水位波动密切相关.水位波动通过改变泥炭孔隙水中微生物的碳代谢功能进而影响泥炭地碳循环.In order to investigate the characteristics of microbial carbon metabolic activity in peatlands with water table fluctuation,microbial community-level physiological profiling of peat pore water was determined with the different water table levels using Biolog-Eco microplate technology in the Dajiuhu Peatland,Shennongjia Forestry District.The results show that the rate and diversity of microbial carbon utilization at the mediate water table were the highest,followed by the low water table,with the lowest at the high water table.Esters(pyruvic acid methyl ester,Tween 40,Tween 80 and D-Galactonic acid-lactone),amino acids(L-arginine,L-asparagine,L-phenylalanine,L-serine and glycyl-L-glutamic acid)and amines(phenylethylamine,putrescine and N-acetyl-D-glucosamine)were the main contributors to the variations in microbial carbon utilization of peat pore water.Redundancy analysis and fitting regression model indicate that electrical conductivity(F=3.2,P=0.018)and oxidation-reduction potential(F=2.6,P=0.044)driven by water table level affected microbial carbon utilization of peat pore water.This study reveals the effect of water table fluctuation on microbial carbon utilization in peat pore water,which enriches our understanding of carbon cycle in peatlands in the context of global climate change.

关 键 词：泥炭地 孔隙水 水位 Biolog⁃Eco微平板 碳代谢 碳循环 环境微生物学. 

分 类 号：P66[天文地球—地质学]