蔓柏生发方改善小鼠的雄激素性脱发可能与肠道菌群相关:基于16S rDNA分析  

Effects of Manbai Shengfa formula on intestinal flora in mice with androgenic alopecia

作　　者：彭丽倩[1,2] 肖常青 王雨诗[3] 李燕红 许端倪 黄久遂[1,2] 梁碧华 赵铁[1,2] 朱慧兰 卢月[3] 李振洁 PENG Liqian;XIAO Changqing;WANG Yushi;LI Yanhong;XU Duanni;HUANG Jiusui;LIANG Bihua;ZHAO Tie;ZHU Huilan;LU Yue;LI Zhenjie(Guangzhou Institute of Dermatology,Guangzhou 510095,China;Institute of Dermatology,Guangzhou Medical University,Guangzhou 510095,China;Second Clinical Medical College,Guangzhou University of Chinese Medicine,Guangzhou 510120,China)

机构地区：[1]广州市皮肤病防治所,广东广州510095 [2]广州医科大学皮肤病研究所,广东广州510095 [3]广州中医药大学第二临床医学院,广东广州510120

出　　处：《皮肤性病诊疗学杂志》2024年第9期577-587,共11页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：广东省基础与应用基础研究基金项目(2019A1515011593);广州市科技计划-市校(院)联合资助基础与应用基础研究项目(2023A03J0948,2024A03J0481,2024A03J0424)。

摘　　要：目的基于16S rDNA技术探索蔓柏生发方对雄激素性脱发(AGA)小鼠的肠道菌群影响。方法将36只C57BL/6小鼠随机分成6组:空白对照组、模型对照组、阳性药物组以及蔓柏生发方高、中、低组,每组6只。剔除所有小鼠脊背部2 cm×3 cm面积的毛发,除空白对照组外,其他组使用丙酸睾酮稀释液在小鼠背部脱毛区域进行皮下多点注射制备AGA模型,每只总剂量为0.1 mg/d。空白对照组和模型对照组在小鼠剃毛处外涂生理盐水,每只1.0 mL/d;阳性药物组涂5%米诺地尔酊,每只1.0 mL/d;蔓柏生发方高、中、低组分别灌胃不同浓度的蔓柏生发方(5.0 g/kg、2.5 g/kg、1.25 g/kg),连续给药30 d。30 d后留取脱毛区皮肤进行病理学观察,收集小鼠粪便进行16S rDNA分析。结果与模型组相比,蔓柏生发方组毛囊数量均明显增多,其中高剂量组可见毛囊呈现生长期样形态,接近空白组毛囊形态。多样性分析结果显示,与空白组比较,模型组肠道菌群丰富度、多样性明显下降,而蔓柏生发方对模型小鼠的肠道菌群丰富度有良好的上调作用,同时其作用具有浓度依赖性,高剂量组对菌群的调控作用最为显著。肠道菌群的α多样性指数在模型组与蔓柏生发方组之间存在显著差异(P<0.05)。结论蔓柏生发方能显著提高AGA小鼠肠道菌群的丰富度和多样性,其对AGA模型小鼠脱毛的治疗作用可能与其调节肠道微生物多样性有关。Objective To investigate the effects of Manbai Shengfa formula on intestinal flora in mice with androgenic alopecia(AGA),using 16S rDNA technology.Methods A total of 36 C57BL/6 mice were randomly divided into 6 groups,i.e.blank control group,model group,positive control group,and high-,medium-,and low-dose of Manbai Shengfa formula,with 6 mice per group.Following removal of hair on the back(2 cm×3 cm area),multiple-point subcutaneous injections of testosterone propionate at a total dose of 0.1 mg/d/mouse were given to establish the AGA model,while the uninjected mice served as blank controls.Afterward,both the blank controls and model mice were treated topically with normal saline(1.0 mL/d/mouse).The positive group was treated topically with 5%minoxidil tincture(1.0 mL/d/mouse).The rest three groups were treated orally with Manbai Shengfa formula at doses of 5.0 g/kg,2.5 g/kg and 1.25 g/kg,respectively,for 30 consecutive days.After 30-day treatments,the skin of the depilated area was collected for pathology,and the feces were collected for 16S rDNA analysis.Results Compared with the untreated model group,Manbai Shengfa formula significantly increased the number of hair follicles.The morphology of hair follicles in the high-dose group was similar to that in the blank controls.The results of diversity analysis showed that the model group exhibited a reduced richness and diversity of intestinal flora compared to the blank controls,while Manbai Shengfa formula dose-dependently increased the richness of intestinal flora in the mouse model of AGA.The alpha diversity indices of intestinal flora differed significantly between the model group and the Manbai Shengfa formula treated groups(P<0.05).Conclusions Manbai Shengfa formula can significantly increase the richness and diversity of intestinal flora.The therapeutic effects of Manbai Shengfa formula for alopecia in AGA model may be linked to the regulation of intestinal microbial diversity.

关 键 词：雄激素性脱发 蔓柏生发方 16S rDNA技术 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]