2021—2022年广州地区鼠形动物中汉坦病毒携带情况及分子流行病学调查  

Prevalence and molecular epidemiology of hantavirus in rodents in Guangzhou City from 2021 to 2022

作　　者：曹毅敏[1] 陆维智 江毅民[1] 梁雪莹[1] 陶霞[1] 伍浩颖[1] 吴继彬[1] 苏文哲[1] 狄飚[1] CAO Yi-min;LU Wei-zhi;JIANG Yi-min;LIANG Xue-ying;TAO Xia;WU Hao-ying;WU Ji-bin;SU Wen-zhe;DI Biao(Guangzhou Municipal Center for Disease Control and Prevention,Guangdong Guangzhou 410440,China)

机构地区：[1]广州市疾病预防控制中心,广东广州510440

出　　处：《江苏预防医学》2024年第4期431-434,共4页Jiangsu Journal of Preventive Medicine

基　　金：广州市医学重点学科(2021-2023-11);广州市卫生健康科技项目(20211A010049);广州市科技计划项目(2023A03J0447)。

摘　　要：目的了解近年广州市鼠形动物中汉坦病毒(Hantavirus,HV)的携带情况。方法对广州市2021—2022年捕获的鼠形动物进行HV携带监测,取肺部组织进行HV核酸检测,对阳性标本进行部分序列测定分析。结果共捕获409只鼠形动物,汉城病毒(Seoul virus,SEOV)阳性检出率为32.27%,未检出汉滩病毒(hantaan virus,HTNV)。SEOV阳性率:褐家鼠36.39%,黄胸鼠30.43%,小家鼠12.50%,臭鼩鼱9.30%,差异有统计学意义(χ^(2)=15.82,P<0.05)。全市11个行政区均有SEOV阳性检出,阳性率8.57%~51.72%,差异有统计学意义(χ^(2)=42.11,P<0.05)。对SEOV阳性标本的部分序列测定和进化树分析结果显示,广州地区的SEOV在进化树中位于同一分支,且与其他研究者在2017年、2019年测得的本地SEOV序列高度同源,均属于S3亚型。结论广州地区鼠形动物中较高的SEOV携带率可能与本地肾综合征出血热的流行高度相关,应对本地鼠形动物进行持续深入的病原携带情况调查和病毒基因组研究。Objective To investigate the prevalence of hantavirus infections in rodents in Guangzhou City in recent years.Methods Rodents were captured from Guangzhou City from 2021 to 2022 and characterized.Rodent lung specimens were collected and tested for hantavirus nuclear acid,and positive lung specimens were subjected to sequencing.Results A total of 409 rodents were captured,and the prevalence of Seoul virus(SEOV)infection was 32.27%,without hantaan virus(HTNV)detected.The prevalence of SEOV infections was 36.39%in Rattus norvegicus,30.43%in Rattus flavipectus,12.50%in Mus musculus and 9.30%in Suncus murinus(χ^(2)=15.82,P<0.05).SEOV was detected in rodents across 11 districts of Guangzhou City,with 8.57%to 51.72%prevalence of infections(χ^(2)=42.11,P<0.05).Sequencing analysis and phylogenetic analysis of SEOV-positive specimens showed that SEOV isolates from Guangzhou City were clustered into the same clade,and shared a high homology with the SEOV sequences in 2017 and 2019,which all belonged to S3 subtype.Conclusions The high prevalence of SEOV infections in Guangzhou City may be strongly associated with the local transmission of hemorrhagic fever with renal syndrome in Guangzhou City.Persistent investigations of hantavirus infections in local rodents and viral genome are recommended in Guangzhou City.

关 键 词：鼠形动物 汉坦病毒 汉城病毒 媒介生物 

分 类 号：S852.65[农业科学—基础兽医学]