术中无标记显微成像技术发展与应用(特邀)  

Developments and applications of intraoperative label-free microscopic imaging techniques(invited)

作　　者：马昊杰 张聪 吴华铮 郭成飞 江劭玮 MA Haojie;ZHANG Cong;WU Huazheng;GUO Chengfei;JIANG Shaowei(Hangzhou Institute of Technology,Xidian University,Hangzhou 311231,China;School of Communication Engineering,Hangzhou Dianzi University,Hangzhou 310018,China;School of Optoelectronic Engineering,Xidian University,Xi’an 710071,China)

机构地区：[1]西安电子科技大学杭州研究院,浙江杭州311231 [2]杭州电子科技大学通信工程学院,浙江杭州310018 [3]西安电子科技大学光电工程学院,陕西西安710071

出　　处：《红外与激光工程》2024年第9期49-68,共20页Infrared and Laser Engineering

基　　金：国家自然科学基金项目(62305258);中国博士后科学基金项目(2023M732731);西安电子科技大学杭州研究院概念验证基金项目(GNYZ2024SJ007);国家资助博士后研究人员计划项目(GZC20232025);中央高校基本科研业务费专项资金项目(ZYTS24008);西安电子科技大学学科交叉拓展特支计划项目(TZJH2024039)。

摘　　要：无标记显微成像技术利用样品的固有特性进行成像,不会破坏生物样本的自身结构与功能,无需耗时繁琐的染色等操作,有利于实现术中快速癌症诊断。首先介绍了不同种类的无标记显微成像技术的成像原理,并讨论了其成像速度与空间分辨率等成像性能,随后介绍了无标记显微成像技术在人类典型癌症诊断中的应用,最后探讨了术中无标记显微成像技术在未来的发展方向。Significance In recent years,numerous researchers have dedicated themselves to developing the label-free microscopic imaging techniques,which rely on the inherent characteristics of biological tissues to provide image contrast and yield image quality comparable to traditional H&E staining.Label-free microscopic imaging techniques offer unique advantages in laboratory and clinical medical research by allowing for the acquisition of detailed tissue and cell structure information without damaging the sample.This technology has shown significant potential in intraoperative cancer diagnosis,enabling surgeons to obtain high-resolution tissue images in real-time and more accurately delineate the boundaries of cancerous lesions,thereby enhancing surgical success rates and patient prognosis.The advancement of this technology not only provides new tools for basic research but also opens new avenues for clinical applications and medical imaging.Progress This review introduces the fundamental principles of several label-free microscopic imaging techniques and summarizes recent advancements in label-free pathological diagnosis across various tumors.Photoacoustic microscopy represents a non-invasive imaging modality that harnesses the photoacoustic effect,integrating the benefits of both optical and acoustic imaging.This technique mitigates the depth limitations inherent in optical imaging while addressing the resolution and contrast constraints associated with acoustic imaging,thereby delivering high-resolution and high-contrast images of deep biological tissues,which is advantageous for cancer diagnosis and evaluation.Optical coherence tomography(OCT)is a high-resolution,non-invasive optical imaging technique extensively utilized in ophthalmology and other medical domains.OCT employs a broadband light source and a Michelson interferometer to capture tomographic images of biological tissues via low-coherence light interference.OCT provides real-time,non-destructive imaging capabilities,with subdivisions including time-domain

关 键 词：显微成像 无标记成像 术中癌症诊断 

分 类 号：O438[机械工程—光学工程]