DNA双链断裂高通量测序技术的研究进展  

High Throughput Sequencing Technologies for DNA Double-Strand Break

作　　者：吴树恒 梁羽 吴薇 WU Shuheng;LIANG Yu;WU Wei(Key Laboratory of Multi-Cell Systems,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,Shanghai 200031,China)

机构地区：[1]多细胞体系结构与功能重点实验室,中国科学院分子细胞科学卓越创新中心/生物化学与细胞生物学研究所,上海200031

出　　处：《中国细胞生物学学报》2024年第8期1488-1496,共9页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:32370575)资助的课题。

摘　　要：DNA双链断裂(DNA double-strand break,DSB)是最严重的DNA损伤类型,研究其产生及修复机制对理解疾病发生、发展具有非常积极的意义。测序技术的发展推动了多种DSB高通量测序技术的诞生,使得在全基因组范围内检测DSB“热点”及其修复加工过程成为可能。目前,这些技术已经广泛应用到DSB研究领域,促进了多个关键修复因子功能的解析及多种体系下断裂热点的鉴定,加深了在染色体环境对DSB调控机制方面的理解,让DSB的研究进入了一个新维度。结合实验室的相关研究内容,该文将对DSB的产生、修复机制及目前已发表的DSB高通量检测技术进行简要概述和总结。The DSB(DNA double-strand break)is the most severe type of DNA damage.Studying the underlying mechanisms of DSB generation and repair will help to understand disease initiation and progression.In the past decade,the advent of next-generation sequencing has greatly promoted the development of sequencingbased methods for DSB detection,making it possible to identify the“hotspots”of DSB and measure the repair and processing events.These assays have been widely applied to investigate the roles of many key repair factors,DSB hotspots in different biological contexts,impact of chromatin context on DSB repair,opening a fascinating portal into DSB research.Combined with the laboratory's research focus,this article will briefly summarize the sources of DSB,their repair pathways,and published genome-wide detection assays for DSB.

关 键 词：DNA损伤修复 DNA双链断裂 高通量测序 

分 类 号：Q754[生物学—分子生物学]