掌叶大黄对寻常型银屑病肠黏膜屏障免疫活性研究  

Study on the Immunomodulatory Activity of Rheum palmatum on the Intestinal Mucosal Barrier in a Mouse Model of Psoriasis Vulgaris

作　　者：黄晶 孙宏伟 HUANG Jing;SUN Hong-Wei(Clinical Medicine Department of Gansu Medical College,Pingliang 744000,Gansu,China)

机构地区：[1]甘肃医学院临床医学系,甘肃平凉744000 [2]甘肃医学院附属医院皮肤科

出　　处：《吉林医学》2024年第10期2328-2331,共4页Jilin Medical Journal

基　　金：甘肃省青年科技基金项目[项目编号:20JR5RA169];2021年甘肃省高等学校创新基金项目[项目编号:2021B-332]。

摘　　要：目的:探讨中药掌叶大黄提取物对寻常型银屑病小鼠模型肠道屏障功能的免疫调节作用,以提供中医药治疗银屑病的新思路和理论依据。方法:使用5%咪喹莫特乳膏诱导联合银屑病人肠道菌群移植诱导形成皮肤病变。试验分为正常组、空白组、阳性药物对照组、大黄干预组,每组15只。除正常组之外,其余三组分别给予咪喹莫特100 mg/次背部涂药,连续8 d,从涂药第3天开始给予银屑病人粪水灌肠。每日造模后,阳性药物对照组给予甲氨蝶呤0.1 mg/ml,大黄干预组给予大黄提取物11 mg灌胃,1次/d,连续给药10 d。银屑病面积与严重性指数(PASI)评分标准对皮损进行评分,酶联免疫吸附试验(ELISA)检测肠黏膜白细胞介素(IL)-17、IL-23水平。结果:PASI评分结果显示,除正常组外,小鼠皮肤在第2天开始出现轻微的损伤。在第10天,小鼠的皮损程度达到顶峰,空白组小鼠的皮损最严重。与空白组相比,大黄干预组与阳性药物对照小鼠的皮肤鳞屑减少,皮肤较平整,PASI评分显著低于空白组,差异有统计学意义(P<0.05),大黄干预组与阳性药物组对照皮损情况差异无统计学意义(P>0.05)。ELISA结果显示,与空白组相比,大黄干预组和阳性药物对照组小鼠的IL-17、IL-23水平显著降低,差异有统计学意义(P<0.01)。结论:掌叶大黄通过调节肠黏膜组织中IL-17、IL-23含量表达,缓解小鼠肠黏膜组织炎性反应,减轻咪喹莫特诱导的银屑病小鼠皮损严重程度,可能是治疗银屑病的机制之一。Objective To investigate the immunoregulatory effects of Rheum palmatum extract on the intestinal barrier function in a mouse model of psoriasis vulgaris,providing new insights and theoretical support for the treatment of psoriasis with traditional Chinese medicine.Method This study established a mouse model of psoriasis vulgaris using 5%clobetasol propionate cream to induce skin lesions.The experiment was divided into a normal group,a blank group,a positive drug control group,and a rhubarb intervention group,with 15 rats in each group.Except for the normal group,the other three groups were given 100 mg/dose of imiquimod for back application for 8 consecutive days.From the third day of application,patients with silver filings were given fecal water enema.After daily modeling,the positive drug control group was given methotrexate 0.1 mg/ml,and the rhubarb intervention group was given rhubarb extract 11mg by gavage once a day for 10 consecutive days.The PASI scoring standard is used to score skin lesions,and the ELISA method is used to detect the levels of IL-17 and IL-23 in the intestinal mucosa.Results The PASI scoring results showed that,except for the normal group,the skin of the mice began to show slight damage on the second day.On the 10th day,the degree of skin lesions in mice reached its peak,with the blank group mice showing the most severe skin lesions.Compared with the blank group,the skin scales of mice in the rhubarb intervention group and the positive drug control group were reduced,the skin was smoother,and the PASI score was significantly lower than that of the blank group,with statistical significance(P<0.05).There was no statistically significant difference in the skin lesions between the rhubarb intervention group and the positive drug control group(P>0.05).The ELISA results showed that compared with the blank group,the levels of IL-17 and IL-23 in the rhubarb intervention group and the positive drug control group mice were significantly reduced,and the difference was statistically significant(P<

关 键 词：掌叶大黄 寻常型银屑病 肠黏膜屏障 免疫调节 

分 类 号：R392[医药卫生—免疫学]