青藤碱调控LncRNA-HEIH表达抑制人食管癌细胞迁移和侵袭  

Sinomenine Inhibits Migration and Invasion of Human Esophageal Cancer Cells by Regulating LncRNA-HEIH Expression

作　　者：陈中凯 伍贤权 敬家辉 龙春晖 李龙 陈伟毅 CHEN Zhongkai;WU Xianquan;JING Jiahui;LONG Chunhui;LI Long;CHEN Weiyi(Medical School of Hunan University of Medicine,Huaihua 418000,Hunan,China)

机构地区：[1]湖南医药学院医学院,湖南怀化418000

出　　处：《医学信息》2024年第19期62-67,共6页Journal of Medical Information

基　　金：国家级大学生创新创业训练计划项目(编号:202212214009);2022年度湖南省大学生创新创业训练计划项目(编号:5021);湖南省自然科学基金项目(编号:2022JJ50290);湖南省卫生健康委科研课题(编号:202104081483)。

摘　　要：目的 探讨青藤碱(SIN)对食管癌细胞迁移、侵袭的影响及对LncRNA-HEIH的调控作用。方法 采用不同浓度青藤碱处理食管癌TE-1细胞,si-NC、si-HEIH分别转染TE-1细胞,pcDNA、pcDNA-HEIH分别转染TE-1细胞后加入青藤碱处理细胞;CCK8检测细胞活力,划痕实验检测细胞迁移率,Transwell实验检测侵袭细胞数,qRT-PCR检测HEIH表达量。结果 青藤碱25、50、100 mg/L对TE-1细胞活力无明显影响(P>0.05),在青藤碱25、50、100 mg/L的作用下,TE-1细胞迁移率和侵袭细胞数显著降低,差异有统计学意义(P<0.01),在青藤碱25、50、100 mg/L的作用下,TE-1细胞HEIH表达显著降低,差异有统计学意义(P<0.01)。转染si-HEIH可显著降低TE-1细胞迁移率和侵袭细胞数,差异有统计学意义(P<0.01),过表达HEIH可显著逆转青藤碱降低TE-1细胞迁移率和侵袭细胞数的作用(P<0.01)。结论 青藤碱通过抑制HEIH表达,抑制食管癌细胞迁移和侵袭。Objective To investigate the effect of sinomenine(SIN)on the migration and invasion of esophageal cancer cells and the regulation of LncRNA-HEIH.Methods Esophageal cancer TE-1 cells were treated with different concentrations of sinomenine.TE-1 cells were transfected with si-NC and si-HEIH,respectively.TE-1 cells were transfected with pcDNA and pcDNA-HEIH,respectively,and then treated with sinomenine.CCK8 was used to detect cell viability,scratch test was used to detect cell migration rate,Transwell test was used to detect the number of invasive cells,and qRT-PCR was used to detect HEIH expression.Results Sinomenine 25,50 and 100 mg/L had no significant effect on the viability of TE-1 cells(P>0.05).Under the action of sinomenine 25,50 and 100 mg/L,the migration rate and invasive cell number of TE-1 cells decreased significantly(P<0.01).Under the action of sinomenine 25,50 and 100 mg/L,the expression of HEIH in TE-1 cells decreased significantly(P<0.01).Transfection of si-HEIH significantly reduced the migration rate and the number of invasive cells of TE-1 cells(P<0.01).Overexpression of HEIH significantly reversed the effect of sinomenine on reducing the migration rate and the number of invasive cells of TE-1 cells(P<0.01).Conclusion Sinomenine inhibits the migration and invasion of esophageal cancer cells by inhibiting the expression of HEIH.

关 键 词：青藤碱 HEIH 迁移 侵袭 食管癌 

分 类 号：R285.5[医药卫生—中药学]