大肠杆菌ABC超家族转运体YadGH药物外排作用及其转运药物的验证  

Drug Efflux of E.coli ABC Superfamily Transporter YadGH and Verification of Transported Drug by YadGH

作　　者：高珊 冯振月 王艳红[1] 崔玉东[1] Gao Shan;Feng Zhenyue;Wang Yanhong;Cui Yudong(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing163319;College of Agronomy and Life Science,Zhaotong University)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]昭通学院农学与生命科学学院

出　　处：《黑龙江八一农垦大学学报》2024年第5期97-103,共7页journal of heilongjiang bayi agricultural university

基　　金：黑龙江八一农垦大学“三横三纵”团队项目支持(TDJH201810)。

摘　　要：大肠杆菌ABC转运体(ABC transporter)能够介导大肠杆菌耐药性,但有关大肠杆菌ABC转运体YadGH介导的耐药性以及转运何种药物尚未见报道。为探究YadGH转运体的外排功能,首先对YadGH进行生物信息学分析,然后采用Red重组技术构建缺失yadG基因的E.coli K-12菌株,并检测多种抗生素对基因缺失菌株的最低抑菌浓度;同时,对表达纯化的YadG蛋白进行ATP酶活性测定。结果显示,yadG基因缺失菌株对于大环内酯类抗生素罗红霉素、泰乐菌素、红霉素的外排转运能力下降,且YadG蛋白具有ATP酶活性。综上,YadGH是一种大环内酯类药物的ABC转运体。ABC transporters mediate drug resistance in Escherichia coli.However,the drug resistance mediated by E.coli ABC transporter YadGH and the type of drug transported has not been reported.To explore the efflux function of YadGH transporter,the bioinformatics analysis of YadGH was carried out.Then yadG gene knockout strains of E.coli K-12 were constructed by Red recombinant technology,and the minimum inhibitory concentration of antibiotics against the yadG gene knockout strains and the ATPase activity of the purified YadG protein were measured.The results showed that the efflux ability of the knockout strains to transport roxithromycin,tylosin,and erythromycin was decreased,and YadG protein showed ATPase activity.The results demonstrated that YadGH was an ABC transporter of macrolide.

关 键 词：大肠杆菌 ABC转运体 YadGH 大环内酯类药物 

分 类 号：Q71[生物学—分子生物学]