基于微流控–免疫荧光层析技术的家庭化胰腺癌检测芯片  

Home-based pancreatic adenocarcinoma test chip based onmicrofluidic chip and immunofluorescence chromatography

作　　者：陈经纬 郑璐璐 CHEN Jingwei;ZHENG Lulu(School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学光电信息与计算机工程学院,上海200093

出　　处：《光学仪器》2024年第5期17-23,共7页Optical Instruments

摘　　要：近年来胰腺癌的高发病率与高致死率引起了人们的广泛关注。现有的检测手段成本高昂且操作复杂,难以推广应用至社区家庭中。而成本较低的传统的免疫层析试纸条难以有效检测血液样本中的标志物。为此,研究设计了一种针对肿瘤标志物CA199的微流控−免疫层析试纸条复合结构,借助光学显微镜,通过芯片实验观察到微流控微阵列滤红结构可以有效分离血液样本中的红细胞,并不会破坏细胞结构。利用透射电子显微镜与多功能酶标仪分析了量子点荧光纳米微球的物理特性,表明其具有良好的稳定性与分散性。与未经滤红样本的对照实验显示了滤红结构对于血液样本中CA199荧光信号的增强作用,系统检测限为4.2 U/mL。微流控−免疫层析试纸条成本低廉,操作简单且安全稳定,为胰腺癌的家庭化筛查提供了一种新的思路。The high incidence and mortality of pancreatic adenocarcinoma have attracted widespread attention in recent years.However,the existing detection methods are costly and complex to operate,which makes it difficult to extend their use to households in the community.On the other hand,the lower-cost traditional immunochromatographic test strips are difficult to detect the markers in blood samples effectively.A microfluidic-immunochromatographic test strip for tumor marker CA199 was designed.With the aid of the optical microscope,the microarray structure could effectively separate red blood cells in sample solution without destroying the cellular structure.The physical properties of the quantum dot fluorescent microspheres were analyzed by a transmission electron microscope and a multifunctional enzyme marker.The study shows that the quantum dot fluorescent microspheres have good stability and dispersity. Bycomparison with unfiltered samples, the enhancement of microfluidic structures for CA199 fluorescent signals in samples was demonstrated. The detection limit of the system is 4.2 U/mL.The microfluidic-immunochromatographic test strips are low-cost, simple-to-operate, safe andstable. It provides a new strategy for home-based screening for pancreatic adenocarcinoma.

关 键 词：微流控芯片 免疫层析技术 肿瘤标志物 胰腺癌 

分 类 号：TB39[一般工业技术—材料科学与工程]