地衣芽孢杆菌对几丁质的降解作用及其发酵工艺优化  

Degradation of Chitin and Optimization of Its Fermentation Process by Bacillus licheniformis

作　　者：罗立津[1] 庄鸿[1] 陈秀明[1] 郑军荣[1] 王秋惠 乐占线[1] 贾纬[1] LUO Lijin;ZHUANG Hong;CHEN Xiuming;ZHENG Junrong;WANG Qiuhui;LE Zhanxian;JIA Wei(Fujian Institute of Microbiology,Fuzhou 350007,China;School of Pharmacy,Fujian Medical University,Fuzhou 350122,China)

机构地区：[1]福建省微生物研究所,福州350007 [2]福建医科大学药学院,福州350122

出　　处：《亚热带资源与环境学报》2024年第3期49-57,共9页Journal of Subtropical Resources and Environment

基　　金：福建省科技计划引导性项目(2020N0019);福建省公益类项目(2022R1005004)。

摘　　要：本试验筛选到一株高效产几丁质酶的地衣芽孢杆菌(Bacillus licheniformis bl3)菌株,为了提高该菌产几丁质酶的能力,提高降解虾壳的效率,对bl3菌株的发酵条件和培养基组分配比进行了优化。结果表明,bl3产几丁质酶的最适条件为pH 4.5~5.5,温度37~45℃,转速120 r·min^(-1),接种量0.40%,酶活性最高时间为36 h。通过Plackett-Burman设计试验、最陡爬坡试验和响应面试验,得到bl3产几丁质酶的最优培养基配方为虾壳11.406 g·L^(-1),KH_(2)PO_(4)0.601 g·L^(-1),MgSO_(4)·7H_(2)O0.263 g·L^(-1),酵母粉3.500 g·L^(-1),FeSO_(4)·2H_(2)O 15.00 mg·L^(-1),ZnSO_(4)0.750 mg·L^(-1)。优化后bl3发酵产几丁质酶活性达98.122 U·mL^(-1),相较于初始培养条件提高2.43倍。优化组合培养基中的虾壳、KH_(2)PO_(4)和MgSO_(4)·7H_(2)O含量以及维持稳定pH,可促进地衣芽孢杆菌持续生长和高效合成几丁质酶量。研究结果可为bl3菌株在降解几丁质方面的应用提供科学依据。A highly potent chitinase-producing strain(Bacillus licheniformis bl3)was selected in this experiment.In order to improve the ability of producing chitinase and the efficiency of shrimp shell degradation,the fermentation medium and fermentation conditions of Bacillus licheniformis bl3 strain were optimized.The experiments showed that the optimal conditions for chitinase production by Bacillus licheniformis bl3 strain were between 4.5~5.5 pH value,37~45℃degree,120 r·min^(-1) speed,inoculum volume was 0.40%,and the highest time of enzyme activation was 36 h.Through Plackett-Burman design test,steepest climbing test and response surface test,the optimal medium formula for bl3 chitinase production were shrimp shell 11.406 g·L^(-1),KH_(2)PO_(4)0.601 g·L^(-1),MgSO_(4)·7H_(2)O 0.263 g·L^(-1),and yeast powder 3.5 g·L^(-1),FeSO_(4)·2H_(2)O 15 mg·L^(-1),ZnSO_(4)0.75 mg·L^(-1).The optimized bl3 fermentation produced chitinase up to 98.122 U·mL^(-1),which was increased to 2.43 times compared with the initial culture conditions.The test results of optimizing the shrimp shell,KH_(2)PO_(4) and MgSO_(4)·7H_(2)O content in the combined medium,and maintaining stable pH to promote the continuous growth and efficient synthesis of chitinase amount can offer a scientific basis for the application of bl3 strains in chitin degradation.

关 键 词：地衣芽孢杆菌 虾壳 几丁寡糖 响应面试验设计 

分 类 号：X713[环境科学与工程—环境工程]