溶藻菌CZBC1在氯化物型盐碱水中对铜绿微囊藻的溶藻效果研究  

Algicidal effect of bacteria CZBC1 on Microcystis aeruginosa in chloride type saline-alkali water

作　　者：胡晓娟[1] 杨铿[1,2,3] 文国樑 苏浩昌[1] 许云娜[1] 徐创文 徐煜[1] 徐武杰 曹煜成[1] HU Xiaojuan;YANG Keng;WEN Guoliang;SU Haochang;XU Yunna;XU Chuangwen;XU Yu;XU Wujie;CAO Yucheng(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300,China;Guangzhou Xinhailisheng Biotechnology Co.,Ltd.,Guangzhou 510300,China;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China)

机构地区：[1]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室/广东省渔业生态环境重点实验室,广东广州510300 [2]广州市欣海利生生物科技有限公司,广东广州510300 [3]中国水产科学研究院东海水产研究所,上海200090

出　　处：《南方水产科学》2024年第5期169-175,共7页South China Fisheries Science

基　　金：国家重点研发计划(2023YFD2401002);广东省重点领域研发计划(2021B0202040001);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2023TD57);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2024XT01);国家现代农业产业技术体系(CARS-48)。

摘　　要：为研发氯化物型盐碱水体溶藻菌菌剂,选取溶藻菌菌株CZBC1,探讨了滤液组、菌体组、菌液组在初始铜绿微囊藻(Microcystis aeruginosa)密度分别为10^(5)和10^(6)个·mL^(-1)的氯化物型盐碱水中对铜绿微囊藻的溶藻效果。结果发现,菌株CZBC1同时具有直接和间接2种溶藻方式,菌株CZBC1的滤液组、菌体组、菌液组作用于铜绿微囊藻时能使藻细胞变形、褪色或破裂,在氯化物型盐碱水体中可以发挥良好的溶藻效果。当初始藻密度为10^(5)个·mL^(-1)时,第6天菌液组的铜绿微囊藻密度可降至2.30×10个·mL^(-1),菌体组降至1.27×10~2个·mL^(-1),对照组为3.67×10^(5)个·mL^(-1),菌液组和菌体组极显著低于对照组(p<0.01),2组的最高溶藻率可达99%以上。当初始藻密度为10^(6)个·mL^(-1)时,第6天菌液组的铜绿微囊藻密度可降至8.30×10^(5)个·mL^(-1),对照组为3.17×10^(6)个·mL^(-1),菌液组显著低于对照组(p<0.05),最高溶藻率为78.8%。从溶藻效果来看,菌液组>菌体组>滤液组。研究表明,溶藻菌CZBC1在氯化物型盐碱水中对铜绿微囊藻具有良好的溶藻效果,可用于氯化物型盐碱池塘中的微囊藻水华防控。In order to develop the algaolytic bacterial agent for chloride type saline-alkali water,we selected the algaolytic bac-terial strain Bacillus cereus CZBC1,and investigated the algaolytic effects of the filtrate group(FG),the bacterium group(BG),and the bacterial solution group(BSG)in the chloride type saline-alkali water with the initial density of Microcystis aeruginosa of 10^(5) and 10^(6) cell·mL^(-1),respectively.The results show that the strain CZBC1 had both direct and indirect ways of dissolving micro-algae.When strain CZBC1 in FG,BG and BSG acted on M.aeruginosa,their microalgal cells could deform,fade or rupture,playing a good role in dissolving algae in chloride type saline-alkali water.When the initial microalgal density was 10^(5) cell·mL^(-1),the microalgal density could be reduced to 2.30×10 cell·mL^(-1) in BSG and 1.27×102 cell·mL^(-1) in BG on the 6th day,which were ex-tremely significantly lower than that in the control group(CG)(3.65×10^(5) cell·mL^(-1),p<0.01),and the highest algicidal rates in both groups could reach over 99%.When the initial microalgal density was 10^(6) cell·mL^(-1),the microalgal density could be re-duced to 8.30×10^(5) cell·mL^(-1) in BSG on the 6th day,which was significantly lower than that in CG(3.17×10^(6) cell·mL^(-1),p<0.05),and its highest algicidal rate could reach over 78.8%.The algicidal effect of BSG was better than that of BG and FG.The results indicate that in order to prevent and control microcystis blooms in chloride type saline alkali ponds,it is recommended to priori-tize prevention and regularly apply algicidal bacteria CZBC1 to prevent and control the formation of harmful algal blooms.

关 键 词：溶藻菌 铜绿微囊藻 蜡样芽胞杆菌 氯化物型盐碱水 

分 类 号：Q939.96[生物学—微生物学]