典型稀土矿区土壤微生物碳循环功能基因组成特征及影响因素研究  

Study on the composition characteristics and influencing factorsof soil microbial carbon cycle functional genesin typical Rare Earth mining area

作　　者：王晋 雷凯能 武小燕 郑春丽[1] WANG Jin;LEI Kaineng;WU Xiaoyan;ZHENG Chunli(Energy and Environment School,Inner Mongolia University of Science and Technology,Baotou 014010,China)

机构地区：[1]内蒙古科技大学能源与环境学院,内蒙古包头014010

出　　处：《内蒙古科技大学学报》2024年第3期205-213,共9页Journal of Inner Mongolia University of Science and Technology

基　　金：国家自然科学基金(42061049);内蒙古科技计划项目。

摘　　要：目前对矿区土壤生态系统碳循环微生物过程的研究有待完善。研究利用宏基因组测序手段,分析了土壤微生物碳循环功能基因的组成特征,并利用相关性分析探究了环境因素对其的影响。结果表明:各样点土壤微生物碳循环功能基因主要由柠檬酸还原循环、二羧酸/4-羟基丁酸酯循环和3-羟基丙酸双循环固碳基因、乙酸发酵型甲烷生成基因及好氧氧化甲烷基因组成。稀土含量与占比最高的微生物碳循环功能基因显著负相关(P<0.05),说明稀土元素减弱了微生物部分碳循环功能。该研究为理解稀土矿区土壤微生物碳循环过程提供了一定的理论支撑。The current research of microbial processes in the carbon cycle of soil ecosystems in mining areas needs to be improved.The aim of this study was to characterize the composition of functional genes associated with the microbial carbon cycle in soil using macro-genome sequencing,and determine the correlation between these genes and environmental factors.The results revealed that the pre-dominant soil microbial carbon cycle functional genes were those related to the citric acid reduction cycle,dicarboxylic acid/4-hydroxy-butyrate cycle,3-hydroxypropionic acid double cycle carbon sequestration genes,acetic acid fermentation-based methanogenesis genes,and aerobic oxidation methane genes.Additionally,correlation analysis identified Rare Earth content,pH,redox potential,and total nitrogen as the primary factors influencing the microbial carbon cycle genes,with Rare Earth content demonstrating a significant nega-tive correlation with the highest proportion of microbial carbon cycle functional genes(P<0.05),suggesting that Rare Earth elements attenuated some of the microbial carbon cycle functions.This study contributes to understanding the theoretical framework for microbial carbon cycling processes in soils from Rare Earth mining areas.

关 键 词：稀土矿区 微生物 碳循环基因 组成特征 

分 类 号：Q93[生物学—微生物学]