猪源盖塔病毒不同感染途径对小鼠致病性的分析  

Analyses of pathogenicity of Getah virus to mice via different infectious pathways

作　　者：段雷雷 蓝建辉 李雯雯 周杭 王伟龙 兰荣招 万根[1,2] 宋德平 DUAN Lei-lei;LAN Jian-hui;LI Wen-wen;ZHOU Hang;WANG Weilong;LAN Rong-zhao;WANG Gen;SONG De-ping(College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi Engineering Research Center for Animal Health Products,Nanchang 330045,China)

机构地区：[1]江西农业大学动物科技学院,江西南昌330045 [2]江西省动物疫病防控制剂工程研究中心,江西南昌330045

出　　处：《中国预防兽医学报》2024年第8期799-804,862,共7页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31970611);江西省自然科学基金重点项目(20232ACB205014)。

摘　　要：为探究猪盖塔病毒(GETV)不同感染途径对小鼠的致病性,及其在小鼠体内各器官和血清中的病毒载量及其排毒情况,本研究将GETV(GETV-JX-CHN-22-P7株)病毒液(106.5TCID50/mL)分别通过点眼、灌胃、腹腔注射3种途径接种小鼠,在96 h的观察期内观察小鼠的临床症状。接种后96 h剖杀各组小鼠,观察其各脏器的剖检病变;采用荧光定量PCR(qPCR)检测各组小鼠各脏器的病毒载量、血清中病毒含量及排毒情况。取各组小鼠的各脏器(脑、肺、心、肝、脾、肾)制备病理切片观察各组织的病理变化。结果显示,3种途径感染GETVJX-CHN-22-P7株均可导致小鼠精神沉郁、食欲下降、活动量减少、轻微发热等症状,其中点眼和灌胃组小鼠感染后96 h的发病率均达100%,腹腔注射组小鼠发病率为60%;所有感染组小鼠均无死亡,对照组小鼠均健活。剖检结果显示,3组感染小鼠的肺、心、脾、肝、肠等脏器均有不同程度的出血,如肺脏出现点状至弥散性出血,脑出血,肝脾肿大及肠淋巴结肿胀等。qPCR结果显示,所有感染组小鼠的眼、脑、肺脏、心脏、脾脏、肾脏及大肠等器官中均可检测到GETV。感染后24 h~96 h,腹腔注射组与灌胃组小鼠的粪便样品中均可检测到GETV,感染后24 h~72 h,点眼组小鼠的粪便样品中均可检测到GETV。腹腔注射组与灌胃组小鼠的眼球、肺、肝、小肠、大肠中的病毒载量均高于点眼组小鼠。组织病理学观察可见,GETV经3种途径感染均可对小鼠的上述各脏器组织造成不同的病理损伤,如脑炎、肝细胞损伤、心肌萎缩坏死、间质性肾炎等病变。上述结果表明,GETV通过点眼、灌胃、腹腔注射3种方式感染小鼠均可引起小鼠相关临床症状;病毒可在小鼠体内多个器官中定植,造成各器官及组织的损伤、病变及病毒血症,并可通过粪便排毒,且不同感染途径与GETV对小鼠的致病性相关。本研究为GETV动物感染�To investigate the pathogenicity of porcine Getah virus(GETV)to mice through different infection routes in mic,and the viral load in various organs and serum and their detoxification,GETV strain GETV-JX-CHN-22-P7(106.5TCID50/mL)was in-oculated into mice by eye drop,oral gavage and intraperitoneal injection.The clinical symptoms of the mice were observed during the 96 hours observation period.The mice in each group were killed 96 hours after inoculation,and the lesions of various organs were observed.Fluorescence quantification PCR(qPCR)was used to analyze the viral load of GETV in the various organs and serum,and detoxification of the mice in each group.Pathological sections were prepared from various organs of the mice in each group,including the brain,lung,heart,liver,spleen,and kidney,to observe the histopathological changes.The results showed that infected via the three routes with the GETV-JX-CHN-22-P7 strain could lead to symptoms such as depression,decreased appetite,reduced activity and mild fever in mice.In particular,both the ocular application and gavage groups had an incidence rate of 100%at 96 hours,while the intraperitoneal injection group had an incidence rate of 60%.None of the infected mice in the treatment groups died,and the control group mice did not exhibit any clinical symptoms or mortality.The necropsy results indicated that varying degrees of hemorrhage were observed in the lungs,heart,spleen,liver,and intestines of the infected mice in all three groups.This included petechial to diffuse pulmonary hemorrhage,cerebral hemorrhage,enlargement of the liver and spleen,and swollen intestinal lymph nodes.qPCR results demonstrated the presence of GETV in the eyes,brain,lungs,heart,spleen,kidneys,and large intestine of all infected group of mice.GETV was detectable in the fecal samples of mice from the intraperitoneal injection and gavage groups from 24 hours to 96 hours post-infection,and in the eye drop group from 24 hours to 72 hours post-infection.The viral load in the eyes,lungs,liver,kidneys,

关 键 词：盖塔病毒 感染途径 病理变化 小鼠 

分 类 号：S852.65[农业科学—基础兽医学]