西番莲属25个种(品种)花粉活力检验方法筛选及其花粉活力和柱头可授性比较分析  

Screening of pollen viability test methods and comparative analysis of pollen viability and stigma acceptability from 25 species of Passiflora L.

作　　者：吴斌 王加志 邢文婷 宋顺 黄海杰[1] 李洪立[1] 胡文斌[1] 马伏宁 黄东梅 WU Bin;WANG Jiazhi;XING Wenting;SONG Shun;HUANG Haijie;LI Hongli;HU Wenbin;MAFuning;HUANG Dongmei(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resourc-es and Germplasm Enhancement in Southern China,Ministry of Agriculture and Rual Affairs/Key Laboratory of Tropical Crops Germ-plasm Resources Genetic Improvement and Innovation of Hainan Province/Germplasm Repository of Passiflora,Hainan Province,Dan-zhou 571101,Hainan,China;College of Tropical Crop Science,Yunnan Agricultural University,Puer 665099,Yunnan,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所·农业农村部华南作物基因资源与种质创制重点实验室·海南省热带作物资源遗传改良与创新重点实验室·海南省西番莲种质资源圃,儋州571101 [2]云南农业大学热带作物学院,云南普洱665099

出　　处：《果树学报》2024年第10期1947-1960,共14页Journal of Fruit Science

基　　金：2024年海南育种联合攻关项目(百香果育种联合攻关);海南省科技计划项目(ZDYF2021XDNY119)。

摘　　要：【目的】探究25种西番莲(包括8个地方品种、15个引进品种/品系和2个选育品种/品系)的花粉活力和柱头可授性,为西番莲杂交亲本的选择提供理论依据。【方法】以25种西番莲为试材,采用花粉离体培养、TTC染色和I2-KI染色方法检测西番莲花粉活力,利用联苯胺-过氧化氢法检测柱头可授性。【结果】25种西番莲均具有柱头可授性,但可授性存在差异。在TN、HX No.1、W-1、KSTS、XG和GH等6种具有代表性的西番莲中,花粉离体培养的最适条件仅蔗糖浓度存在差异,分别为10%和15%。花粉离体培养和TTC染色法均可用于25种西番莲花粉活力的检测。基于花粉活力的聚类分析可分为3个类群,其中第Ⅰ类群和第Ⅱ类群共22个种,均属于正常可育类型,第Ⅲ类群包含3个种,均属于高不育类型。【结论】花粉离体培养是最能呈现25种西番莲花粉活力的检测方法,TTC染色法可作为西番莲花粉活力的快速检测方法。除ZL、LX和RX适宜作杂交母本外,其余种均可作杂交父本或母本。【Objective】The study aimed to test the pollen viability and stigma receptivity of 25 species[including 8 local varieties are Tainong(TN),Xintainong(XTN),Qinmi No.9(QM No.9),Fengmihuangjinbaixiangguo(FMHJ),Yuanyangmi(YYM),Juwubabaixiangguo(D1-A1),Tianhuangxing(THX),Zhuangxiangmibao(ZXMB),15 introduced varieties/strains are Guanhua(GH),Kangsitansi(KSTS),W-1,Honghuaxifanlian(XG),Jingchixifanlian(MGMX),Ruixiang(RX),Zilian(ZL),Zhangyexifanlian(JMCH),Lüpi(LP),Qingpi(QP),Hongguan(HG),ZZ-B1,Lüpixifanlian(Ⅱ-Y),Wulaguireqingguo(ULGRQG),Lanxiang(LX),and 2 selected varieties/strains are Huangxiang No.1(HX No.1),Xiaohuagnjin(D2-11).]of Passiflora L.,and provide theoretical basis for the selecting parents for crossing in Passiflora L.【Methods】Using six representative species of Passiflora L.as test materials,including TN,HX No.1,GH,W-1,XG and KSTS a single-factor experiment was conducted using a basic medium containing 0.02%boric acid(H_3BO_3),15%sucrose,15%PEG-4000,0.08%calcium nitrate[Ca(NO_3)_2·4H_2O],and 0.02%MgSO_4·7H_2O to screen optimal conditions for pollen viability test in vitro.The 25 species of Passiflora L.were used as materials,the basic floral morphological parameters were measured.Pollen vitality was detected using pollen culture in vitro,TTC staining,and I2-KI staining methods to compare differences of pollen vitality of different species with different detection methods.Additionally,the stigmatic receptivity of the 25 species of Passiflora L.was compared and analyzed using the benzidine-hydrogen peroxide method.【Results】The results showed that all the 25 species of Passiflora L.exhibited different stigma receptivity.Among them,the 19 species including TN,HX No.1,W-1,XG,XTN,QM No.9,MGMX,LP,QP,FMHJ,HG,YYM,ZZ-B1,D1-A1,THX,ZXMB,D2-11,Ⅱ-Y and ULGRQG exhibited strong stigma receptivity.The 3 species,GH,KSTS and JMCH had moderate stigma receptivity,while another 3 species,RX,ZL and LX showed weaker stigma receptivity.Only the optimal sucrose concentration for different species was different in po

关 键 词：西番莲属 花粉活力 柱头可授性 杂交育种 

分 类 号：S667.9[农业科学—果树学]