有机肥配施赤霉素和混合芽孢杆菌对柑橘黄龙病的作用  

Effects of organic fertilizer combined with gibberellin and mixed Bacillus subtilis on citrus Huanglongbing

作　　者：王立 刘颖 张逸东 魏文康 刘勤坚 陈华胜 罗敏 俞婷 WANG Li;LIU Ying;ZHANG Yi-dong;WEI Wen-kang;LIU Qin-jian;CHEN Hua-sheng;LUO Min;YU Ting(Agricultural Biological Gene Research Center,Guangdong Academy of Agricultural Sciences,Guangdong Provincial Key Laboratory for Crop Germplasm Resources Preservation and Utilization,Guangzhou Guangdong 510640;Xinhui Forestry Science Institute of Jiangmen City,Jiangmen Guangdong 529100;Lianjiang Association of Economic and Social Development Research,Zhanjiang Guangdong 524400)

机构地区：[1]广东省农业科学院农业生物基因研究中心,广东省农作物种质资源保存与利用重点实验室,广东广州510640 [2]江门市新会区林业科学研究所,广东江门529100 [3]廉江市经济社会发展研究会,广东湛江524400

出　　处：《中国土壤与肥料》2024年第8期221-232,共12页Soil and Fertilizer Sciences in China

基　　金：广东省重点领域研发计划项目(2020B0202090005);江门市科技计划项目(2021030101240005136);企业科技特派员项目(GDKTP2021055300)。

摘　　要：比较有机肥配施微生物菌剂、赤霉素对柑橘黄龙病菌的含量、土壤有机质及土壤微生物群的影响,为柑橘黄龙病的治疗和柑橘土壤肥力提升提供科学依据。从柑橘叶片内生菌中分离得到枯草芽孢杆菌,设置有机肥配施复合芽孢杆菌剂、25 mg/L赤霉素的病株为处理组,另一组为对照,对柑橘黄龙病菌含量、土壤有机质及土壤微生物进行比较分析。与对照组相比,有机肥配施复合芽孢杆菌、赤霉素处理柑橘的带菌量显著下降,黄龙病在荧光定量PCR扩增中从基线到指数增长的拐点所对应的循环次数(Ct值)从初始的25.10增加到了31.46,土壤有机质和速效钾分别提高了22.0%和102.0%。复合芽孢杆菌分泌出的代谢产物既能抑制病菌的生长,还能通过建立优势益生菌群优化土壤结构,促进根系生长,有效解决营养输送的问题,恢复病树状态。有机肥配施复合芽孢杆菌和赤霉素对改善柑橘黄龙病,提高土壤有机质、速效钾等有显著促进作用。The effects of combined application of organic fertilizer with mixed Bacillus subtilis and gibberellin on the content of Candidatus Liberibacterasiaticus(CLas),soil organic matter and soil microorganisms were compared in order to provide a scientific basis for the treatment of Huanglongbing and the improvement of citrus soil fertility.The Bacillus subtilis was isolated from endophytic bacteria in citrus leaves.The organic fertilizer combined with mixed Bacillus subtilis and 25 mg/L gibberellin treatment was used,while the other group doing without any treatment was used as the control.The contents of CLas,soil organic matter and soil microorganisms were compared and analyzed.Compared with the control group,the treatment group reduced CLas significantly,and the Ct value increased from the initial 25.10 to 31.46,soil organic matter and available potassium increased by 22.0%and 102.0%,respectively.The metabolites secreted by Bacillus complex could not only inhibit the growth of bacteria,but also optimize the soil structure by establishing dominant probiotics,promote root growth,effectively solve the problem of nutrient transport,and restore the state of diseased trees.Combined application of organic fertilizer with mixed Bacillus subtilis and gibberellin could significantly promote the improvement of citrus Huanglongbing,soil organic matter and available potassium.

关 键 词：红江橙 茶枝柑 黄龙病 植物内生细菌 芽孢杆菌 赤霉素 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]