超高效液相色谱-串联质谱法测定动物源性食品中4种β-阻断剂残留  

Determination of four β-receptor blockers in animal originated food by ultra-high performance liquid chromatography-tandem mass spectrometry

作　　者：姚静 吴恋 刘川 石培育 廖梅 毛锐 陈世奇 宋娟 YAO Jing;WU Lin;LIU Chuan;SH Pei-yu;LIAO Mei;MAO Rui;CHEN Shi-qi;SONG Juan(Chengdu Institute of Food Inspection,Chengdu 611130,China;Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation,Beijing 100029,China;Key Laboratory of Condiment Supervision Technology for State Market Regulation,Chongqing 401120,China)

机构地区：[1]成都市食品检验研究院,四川成都611130 [2]国家市场监管重点实验室(营养与健康化学计量及应用),北京100029 [3]国家市场监管重点实验室(调味品监管技术),重庆401120

出　　处：《化学研究与应用》2024年第10期40-47,共8页Chemical Research and Application

基　　金：国家重点研发计划课题项目(2022YFF0710405)资助;四川省重点研发项目(2022YFS0511)资助;国家市场监管重点实验室(调味品监管技术)开放课题项目(KLCST-2022-03)资助。

摘　　要：建立了同位素标记-超高效液相色谱-串联质谱法测定畜肉、禽肉、鱼肉、牛奶和鸡蛋中普萘洛尔、美托洛尔、卡替洛尔、阿替洛尔4种β-阻断剂残留的检测方法。样品酶解后经乙腈提取,通过式固相萃取柱净化,超高效液相色谱-串联质谱仪测定,同位素内标法定量。结果表明,4种β-阻断剂在相应质量浓度范围内线性关系良好,相关系数均大于0.995,检出限为0.5μg·kg^(-1),方法回收率在78.4~102.6%之间,相对标准偏差(n=6)为1.9~8.7%。该方法简便快速,灵敏度高,实用性强,可用于动物源性食品中4种β-阻断剂残留检测。A method for the determination of propranolol、metoprolol,carteolol and atenolol inmeat、poultry fish,milk andeggswas established by isotope labeling-ultra-high performance liquid chromatography-tandem mass spectrometry.The sample was extracted with acetonitrile after enzymatic hydrolysis,and the extract was purified by pass-through solid phase extraction before being meas-ured by liquid chromatography-tandem mass spectrometry.The internal standard method was used for quantification.The results showed that good linear relationships were obtained for four ofβ-receptor blockers in the investigated concentration ranges with cor-relation coefficients more than 0.995.The limits of detection(LODs)were 0.5μg·kg^(-1).Average recoveries of the analytes in pork ranged from 78.4~102.6%,with relative standard deviations(RSDs)of 1.9~8.7%.The developed method was simple,rapid and practical,and could be used for the determination of fourβ-receptor blockers in animal originated food.

关 键 词：超高效液相色谱-串联质谱 同位素标记 动物源性食品 Β-阻断剂 

分 类 号：O623.42[理学—有机化学]