贝莱斯芽孢杆菌TCS001悬浮剂配方优化及对草莓炭疽病的防治效果  

Optimization of the formulation for Bacillus velezensis TCS001 suspension and its control efficacy on strawberry anthracnose

作　　者：曹灏 陈智磊 李雪松 宋格宁 李忠 崔峰 陈杰 CAO Hao;CHEN Zhilei;LI Xuesong;SONG Gening;LI Zhong;CUI Feng;CHEN Jie(National and Local Joint Engineering Laboratory for Efficient Preparation of Biopesticides,College of Forestry and Biotechnology,Zhejiang A&F University,Lin’an 311300,Zhejiang Province,China;Zhejiang Tonglu Huifeng Biosciences Co.,Ltd.,Tonglu 311500,Zhejiang Province,China)

机构地区：[1]浙江农林大学林业与生物技术学院生物农药高效制备技术国家地方联合工程实验室,浙江临安311300 [2]浙江省桐庐汇丰生物科技有限公司,浙江桐庐311500

出　　处：《农药学学报》2024年第5期911-921,共11页Chinese Journal of Pesticide Science

基　　金：浙江省重点研发计划(2021C02046)。

摘　　要：贝莱斯芽孢杆菌Bacillus velezensis TCS001是一种新型的海洋生防细菌,具有广谱抑菌活性和促进植物生长的作用,在农业实际应用中具备开发潜力。本研究采用含毒介质法、生长量测定法以及悬浮性能评价的方法,筛选了与TCS001相容性高的润湿分散剂、增稠剂、防腐剂等助剂及其配比,所得TCS001悬浮剂(TCS001 SC)最佳配方组成为:40%TCS001发酵液(≥10^(10)CFU/mL)、3%润湿分散剂SXC、3%润湿分散剂UNA、0.15%增稠剂黄原胶、0.2%防腐剂卡松、0.2%防腐剂苯甲酸钠,水补足至100%(均为质量分数)。其活菌数为4.0×10^(9)CFU/mL、杂菌率为4.8%、pH值为6.68、悬浮率为97.8%、75μm试验筛通过率为99.9%、倾倒后残余物质量分数为1.6%、洗涤后残余物质量分数为0.41%、持久起泡性(1 min后)为16 mL,各项性能均符合行业标准(NY/T 2989-2016)。TCS001 SC在不同温度下的储存稳定性测定结果显示:(0±0.2)℃储存7 d、(4±0.2)℃储存360 d活菌数均较储前无明显差异,(28±0.2)℃储存360 d、(35±0.2)℃储存84 d菌体存活率均在80%以上,表明该制剂储存稳定性较好。在此基础上,以草莓炭疽病为靶标,开展田间药效试验,结果发现,TCS001 SC1500~3000 mL/hm~2喷雾处理可以显著减轻草莓炭疽病的发生,防治效果在59.37%~79.52%之间。其中3000 mL/hm~2间隔7 d,连续喷雾3次对草莓炭疽病的防治效果达到79.52%,该结果可为TCS001 SC用于登记防治草莓炭疽病的微生物杀菌剂提供理论基础。Bacillus velezensis TCS001 is a new type of marine biocontrol bacterium,which has broad-spectrum antifungal activity and promotes plant growth,and has great potential for development in agricultural applications.In this study,the additives and proportions of wetting and dispersing additives,thickeners,and preservatives with high compatibility with TCS001 were screened using the toxic medium method,growth determination method and suspension performance evaluation method,and the formula of TCS001 suspension agent(TCS001 SC)was obtained.The screening results showed that the TCS001 SC formula was:40%TCS001 fermentation broth(≥1010 CFU/mL),3%wetting and dispersing additives SXC,3%wetting and dispersing additives UNA,0.15%thickener xanthan gum,0.2%preservative Kathone,0.2%preservative sodium benzoate.When the water was supplemented to 100%(mass fraction),the number of viable bacteria of TCS001 SC developed was 4.0×10^(9) CFU/mL,with a miscellaneous bacteria rate of 4.8%.For TCS001 SC,the pH value was 6.68,and the suspension rate was 97.8%.The pass rate through the 75μm test sieve was 99.9%,and the mass fraction of residue after pouring was 1.6%,and after washing was 0.41%.And the long-lasting foaming property(after 1 min)was 16 mL,all performances are in line with industry standards(NY/T 2989—2016).Further more,the storage stability of TCS001 SC was measured at different temperatures,there was no significant difference in the number of viable bacteria when stored at(0±0.2)℃for 7 days and(4±0.2)℃for 360 days compared to before storage respectively,and the survival rate of bacterial cells was above 80%when stored at(28±0.2)℃for 360 days and(35±0.2)℃for 84 days,respectively indicating good storage stability.On this basis,the field test was carried out with strawberry anthracnose as the target,and the results showed that TCS001 SC 1500-3000 mL/hm^(2) could significantly reduce the occurrence of strawberry anthracnose,and the control efficacy was between 59.37%-79.52%.Among them,the control efficacy of 300

关 键 词：贝莱斯芽孢杆菌 悬浮剂 配方筛选 稳定性 草莓炭疽病 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.68[农业科学—植物保护]