新疆和田县核桃腐烂病菌鉴定及田间生防制剂筛选  

Identification of walnut rot pathogen and screening of field biocontrol agents in Hetian County,Xinjiang

作　　者：王文窈 孙洪涛 王莉莉 祖里皮也·买提卡斯木 买买托合提·吐孙 马荣[1] WANG Wenyao;SUN Hongtao;WANG Lili;ZULIPIYE·Maitikasimu;MAIMAITUOHETI·Tusun;MA Rong(Collage of Forestry and Landscape Architecture,Xinjiang Agricultural University,Urumqi 830052,China;Forestry and Grassland Bureau of Hetian Prefecture,Hetian 848000,Xinjiang Uygur Autonomous Region,China;Hetian County Forestry and Grassland Bureau,Hetian Prefecture,Hetian 848000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆农业大学林学与风景园林学院,乌鲁木齐830052 [2]新疆维吾尔自治区和田地区林业和草原局,新疆维吾尔自治区和田848000 [3]新疆维吾尔自治区和田地区和田县林业和草原局,新疆维吾尔自治区和田848000

出　　处：《农药学学报》2024年第5期983-993,共11页Chinese Journal of Pesticide Science

基　　金：新疆维吾尔自治区重点研发专项(2021B02004-3)。

摘　　要：为明确引起核桃腐烂病的病原菌种类,通过组织分离及致病性回接,确定致病菌,依据形态学特征结合分子生物学方法完成病菌的种类鉴定;比较3种免疫诱抗剂喷淋以及5种生物制剂与2种助剂刮治对核桃腐烂病的防治效果。从采自和田县的14份病枝样品中分离出15个菌株,其中14株为金黄壳囊孢(Cytospora chrysosperma),1株为普尔曼隐球菌(Cryptosphaeria pullmanensis),表明C.chrysosperma为和田县核桃腐烂病主要致病菌。3种免疫诱抗剂中0.5%几丁聚糖AS 200倍液和5%氨基寡糖素AS 500倍液的诱导效果较好,防治效果分别为51.43%和45.47%。生物制剂中1000亿CFU/g解淀粉芽孢杆菌WP700倍液+99%绿颖矿物油EC 200倍液和3%中生菌素AS 500倍液+99%绿颖矿物油EC 200倍液的防治效果较好,分别为92.31%和84.61%。本研究明确了和田县核桃腐烂病的病原菌,并为核桃腐烂病的田间生物防控提供了参考。In order to identify the pathogenic bacteria causing walnut rot disease,the pathogenic bacteria were identified through tissue isolation and pathogenicity tie-back,and the species identification was completed based on morphological characteristics combined with molecular biology.Furthermore,the effects of three types of immune-inducers spray as well as five types of biologicals and two types of adjuvants scraping were compared on walnut rot.Fifteen strains were isolated from 14 diseased branch samples,of which 14 strains were identified morphologically and molecularly as Cytospora chrysosperma,and one strain was Cryptosphaeria pullmanensis.Therefore,the main causal agent of walnut rot disease in Hetian County was C.chrysosperma.Among the three kinds of immune-inducing antagonists,0.5%chitosan AS 200×and amino-oligosaccharide AS 500×showed better-inducing effects of 51.43%and 45.47%,respectively.Among the biological agents,the control effects of 100 billion CFU/g Bacillus amyloliquefaciens WP 700×+99%SK Enspray 99 EC 200×and 3%Zhongshengmycin AS 500×+99%SK Enspray 99 EC 200×were better,92.31%and 84.61%,respectively.This study clarified the pathogen of walnut rot disease in Hetian County and provided a reference for the biological control of walnut rot disease in the field.

关 键 词：核桃腐烂病 病原鉴定 免疫诱抗剂 生物制剂 田间防治效果 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]