基于细胞膜色谱技术的鹿角胶肽段筛选及鉴定  

To explore the pharmacodynamic substance basis of Cervi Cornus Colla based on cell membrane chromatography

作　　者：孙铁锋[1] 赵玉[2] 王平[1] 丁相龙 丁立钧 王金国 SUN Tiefeng;ZHAO Yu;WANG Ping;DING Xianglong;DING Lijun;WANG Jinguo(Shandong Academic of Chinese Medicine,Jinan 250014,China;Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Huangdao District Second Hospital of Traditional Chinese Medicine,Qingdao 266400,China;Rizhao Traditional Chinese Medicine Hospital,Rizhao 276800,China)

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学,山东济南250355 [3]山东省青岛市黄岛区第二中医医院,山东青岛266400 [4]山东省日照中医院,山东日照276800

出　　处：《山东科学》2024年第5期1-9,94,共10页Shandong Science

基　　金：济南市“新高校20条”(202333006);山东省中医药科技项目(2020Q023);山东省自然科学基金(ZR2020MH386);国家重点研发计划政府间国际科技创新合作项目(2019YFE0117800);中央引导地方科技发展专项基金(YDZX2021117);山东省重点研发计划(科技示范工程)2021SFGC1205;山东省中央引导地方科技发展资金项目(YDZX20203700002055)。

摘　　要：利用细胞膜色谱/超高相液相色谱-质谱(CMC/UPLC-MS)方法对中药饮片鹿角胶中的有效成分进行筛选,结合Maxquant软件、Perseus软件以及Uniprot数据库对所得谱图进行数据分析,使用Protein Data Bank对分析结果进行结构鉴定,同时采用生物信息学平台对这些肽段的生物活性、不良反应、相对分子质量、等电点、不稳定指数等基本分子特性进行预测。鉴定肽段活性概率为0.09,无毒,无溶血性,有致敏性,相对分子质量为1541.68,肽链长度为14,亲水性为良好,等电点值为3.92,不稳定性指数为34.39。该方法为快速筛选鉴定具有药理作用的多肽和蛋白质等活性成分提供了一条可行的研究思路。Cell membrane chromatography/ultrahigh-phase liquid chromatography-mass spectrometry(CMC/UPLC-MS)was used to screen the active ingredients,i.e.,peptides,in the traditional Chinese medicine tablet Cervi cornus Colla,and the obtained spectra were analyzed using Maxquant software,Perseus software,and Uniprot database.Structures of these peptides were identified using Protein Data Bank,and their molecular properties such as their biological activity,adverse reactions,relative molecular mass,isoelectric point,and stability index were predicted using a bioinformatics platform.With an activity probability of 0.09,the peptides were identified as nontoxic,nonhemolytic,sensitizing,and highly hydrophilic,with a relative molecular mass of 1541.68,a peptide chain length of 14,an isoelectric point of 3.92,and an instability index of 34.39.This method provides a feasible research approach for rapidly screening and identifying active ingredients(e.g.,peptides and proteins)that exhibit pharmacological effects.

关 键 词：鹿角胶 细胞膜色谱 骨髓间充质干细胞 白细胞介素-1Β 

分 类 号：R284[医药卫生—中药学]