蚌埠市6~15岁儿童急性呼吸道感染病原谱检测分析  

Analysis of pathogens of acute respiratory infections in children aged 6-15 in Bengbu City

作　　者：付广林[1] 许静茹 董娟[1] 钱青文[1] 梁寅生 吴珊珊 FU Guanglin;XU Jingru;DONG Juan;QIAN Qingwen;LIANG Yinsheng;WU Shanshan(Department of Microbial Detection,Bengbu Center for Disease Control and Prevention,Bengbu,Anhui 233080,China)

机构地区：[1]安徽省蚌埠市疾病预防控制中心微生物检验科,233080 [2]安徽省蚌埠市第一人民医院公共卫生科,233000

出　　处：《淮海医药》2024年第5期445-449,共5页Journal of Huaihai Medicine

基　　金：蚌埠市科技计划(2022hm02)。

摘　　要：目的:分析蚌埠市6~15岁儿童急性呼吸道感染病原谱特征,为制定有效防控措施提供依据。方法:2022年1—8月,采集蚌埠市流感监测哨点医院流感样病例咽拭子标本1 625份,其中6~15岁儿童标本233份,每月抽取10例左右6~15岁儿童病例标本,共抽取80例。使用荧光定量PCR法进行流感病毒和新冠病毒核酸检测,应用FilmArray多重巢式PCR检测技术检测22种病原体。分析流感、新冠病毒实时荧光定量PCR检测结果,以及蚌埠市6~15岁儿童急性呼吸道感染病原体种类、不同月份急性呼吸道感染病原体构成、不同年龄段及性别急性呼吸道感染病原体检出情况、病原体检出特征。结果:1 625份送检标本,新冠检测全部阴性;流感检出阳性242例(14.89%),其中甲型季节性H3亚型阳性133例(8.18%),B型Victoria系阳性109例(6.71%)。233份6~15岁儿童标本,流感检出阳性23例(9.87%),其中甲型季节性H3亚型阳性4例(1.72%),B型Victoria系阳性19例(8.15%)。6~15岁儿童与全部人群的流感及亚型检出率比较差异无统计学意义(P>0.05)。80份呼吸道咽拭子标本中检出病原体28份(35.00%);检出7种病原体共32株,其中人鼻病毒/肠道病毒(37.50%)、B型流感病毒(34.38%)占比较高。2022年1—2月是B型流感流行高峰,其他病原体检测阳性率不同月份间比较,差异均无统计学意义(P>0.05)。不同年龄段、性别急性呼吸道感染病原体检出阳性率比较,差异无统计学意义(P>0.05)。28例检测阳性病例标本中,单一感染25例(89.29%),双重感染2例(7.14%),三重感染1例(3.57%),单一感染占比最高(P<0.001);检出病毒感染26例(92.86%),细菌2例(7.14%)。结论:人鼻病毒/肠道病毒和流感病毒是蚌埠市6~15岁儿童主要的急性呼吸道感染病原体,除流感病毒外,人鼻病毒等其他病原体不同时期检测阳性率比较,差异均无统计学意义。Objective:To explore the pathogen spectrum composition of acute respiratory infections among primary and secondary school students in Bengbu City,and to provide basis for formulating effective prevention and control measures.Methods:From January to August 2022,1625 throat swab samples of influenza like cases were collected from the influenza monitoring sentinel hospital in Bengbu City,including 233 samples from children aged 6-15 years old.About 10 samples from children aged 6-15 years old were collected each month,totaling 80 samples.FilmArray multiple nested PCR was used to detect 22 pathogens,and fluorescent quantitative PCR was used to detect influenza virus and COVID-19 nucleic acid.Analysis was conducted in terms of the types of pathogens of acute respiratory infection in children aged 6 to 15 years old in Bengbu,the composition of pathogens of acute respiratory infection in different months,the physical examination of pathogens of acute respiratory infection in different age groups and sexes,the characteristics of pathogens,and the real-time fluorescent quantitative PCR detection results of influenza and COVID-19.Results:28 pathogens were detected out of 80 respiratory throat swab specimens(35.00%).A total of 32 strains of 7 pathogens were detected,among which rhinovirus/enterovirus(37.50%)and influenza B virus(34.38%)accounted for a relatively high proportion.January and February 2022 were the peak of the B-type influenza epidemic,and there was no statistically significant difference(P>0.05)in the positive rates of other pathogens detected between different months.There was no statistically significant difference(P>0.05)in the positive rate of acute respiratory infection pathogens among different age groups and genders.Among the 28 positive cases detected,25 cases(89.29%)were single infections,2 cases(7.14%)were double infections,and 1 case(3.57%)was triple infections,with the highest proportion of single infections(P<0.001).26 cases(92.86%)of viral infection and 2 cases(7.14%)of bacterial infection were

关 键 词：呼吸道感染 FilmArray多重巢式PCR 病原体 人鼻病毒/肠道病毒 流感病毒 

分 类 号：R517.6[医药卫生—内科学]