子宫肌瘤组织中miR-203a-3p及CBX8表达水平及其临床意义  

Expression levels and clinical significance of miR-203a-3p and CBX8 in uterine leiomyoma

作　　者：陈小兰 雷霞 皮发利 CHEN Xiaolan;LEI Xia;PI Fali(Department of Obstetrics and Gynecology,Dongxing District People′s Hospital of Neijiang,Neijiang,Sichuan 641100,China)

机构地区：[1]四川省内江市东兴区人民医院妇产科,四川内江641100

出　　处：《检验医学与临床》2024年第20期2958-2964,共7页Laboratory Medicine and Clinic

基　　金：四川省卫生健康委员会科研课题(19PJ021)。

摘　　要：目的探究子宫肌瘤组织中微小RNA(miR)-203a-3p及染色体结合蛋白8(CBX8)表达水平及其临床意义。方法选取2019年6月至2021年6月该院收治的112例子宫肌瘤患者作为研究对象,采用实时荧光定量聚合酶链反应检测子宫肌瘤组织与肌瘤旁组织miR-203a-3p、CBX8 mRNA表达水平,免疫组织化学法检测CBX8蛋白表达。以子宫肌瘤组织中miR-203a-3p表达的中位数为界,将子宫肌瘤患者分为miR-203a-3p低表达组和miR-203a-3p高表达组。以子宫肌瘤组织中CBX8蛋白免疫组化染色的评分为界,将子宫肌瘤患者分为CBX8蛋白阴性表达组和阳性表达组。在Targetscan数据库中进行miR-203a-3p、CBX8靶向关系查询。对112例子宫肌瘤患者术后进行2年随访,根据随访结果将患者分为复发组与未复发组。采用Pearson相关分析子宫肌瘤组织miR-203a-3p表达水平与CBX8 mRNA表达水平的相关性。采用多因素Logistic回归分析子宫肌瘤患者术后复发的影响因素。结果子宫肌瘤组织中miR-203a-3p表达水平明显低于肌瘤旁组织,CBX8 mRNA表达水平明显高于肌瘤旁组织,差异均有统计学意义(P<0.05)。CBX8蛋白阳性率(45.54%,51/112)明显高于肌瘤旁组织(21.43%,24/112),差异有统计学意义(χ^(2)=14.613,P<0.05)。Pearson相关分析结果显示,子宫肌瘤组织中miR-203a-3p表达水平与CBX8 mRNA表达水平呈负相关(r=-0.562,P<0.05)。Targetscan数据库检索结果显示,miR-203a-3p与CBX8存在特异性结合位点。miR-203a-3p低表达组、高表达组均纳入56例患者。miR-203a-3p低表达组与高表达组肿瘤最大径、肌瘤数目、生殖道炎、月经紊乱情况比较,差异均有统计学意义(P<0.05)。CBX8蛋白阴性表达组纳入61例患者,阳性表达组纳入51例患者。CBX8蛋白阴性表达组、阳性表达组肿瘤最大径、肌瘤数目、生殖道炎、月经紊乱情况比较,差异均有统计学意义(P<0.05)。子宫肌瘤患者随访率为100%,术后复发组纳入41例患者,�Objective To investigate the expression levels and clinical significance of microRNA(miR)-203a-3p and chromosome binding protein 8(CBX8)in uterine leiomyoma.Methods A total of 112 patients with uterine leiomyoma admitted to the hospital from June 2019 to June 2021 were selected as the research objects.The expression levels of miR-203a-3p and CBX8 in uterine leiomyoma tissues and adjacent leiomyoma tissues were detected by real-time fluorescence quantitative polymerase chain reaction,and the expression of CBX8 protein was detected by immunohistochemistry.According to the median expression of miR-203a-3p in uterine leiomyoma tissues,the patients with uterine leiomyoma were divided into miR-203a-3p low expression group and miR-203a-3p high expression group.According to the score of CBX8 protein immunohistochemical staining in uterine leiomyoma tissues,the patients with uterine fibroids were divided into CBX8 protein negative expression group and CBX8 protein positive expression group.The Targeting relationship between miR-203a-3p and CBX8 mRNA was queried in the Targetscan database.A total of 112 patients with uterine fibroids were followed up for 2 years after operation.According to the follow-up results,the patients were divided into recurrence group and non-recurrence group.Pearson correlation was used to analyze the correlation between the expression levels of miR-203a-3p and CBX8 mRNA in uterine leiomyoma tissue.Multivariate Logistic regression was used to analyze the influencing factors of postoperative recurrence of uterine fibroids.Results The expression level of miR-203a-3p in uterine leiomyoma tissues was significantly lower than that in adjacent leiomyoma tissues,and the expression level of CBX8 was significantly higher than that in adjacent leiomyoma tissues,and the differences were statistically significant(P<0.05).The positive rate of protein in CBX8(45.54%,51/112)was significantly higher than that in adjacent leiomyoma tissues(21.43%,24/112),and the difference was statistically significant(χ^(2)=14.6

关 键 词：子宫肌瘤 微小RNA-203a-3p 染色体结合蛋白8 预后 相关性 

分 类 号：R737.33[医药卫生—肿瘤] R446.6[医药卫生—临床医学]