Microencapsulated Lactobacillus plantarum promotes intestinal development through gut colonization of layer chicks  

作　　者：Yaoming Cui Yanxia Liu Jing Yang Haitao Duan Peng Wang Linna Guo Yanjiao Guo Suying Li Yating Zhao Jinrong Wang Guanghai Qi Junjun Guan 

机构地区：[1]School of Biological Engineering,Henan University of Technology,Zhengzhou,Henan 450001,China [2]College of Animal Science and Technology,Henan University of Animal Husbandry and Economy,Zhengzhou,Henan 450046,China [3]Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Animal Nutrition》2024年第3期1-16,共16页动物营养（英文版）

基　　金：financed by the National Key Research and Development Program of China (2021YFD1300300);the National Natural Science Foundation of China (32202697);Key Technology Research and Development Program of Henan Province (232102111041);the Innovation Fund of Henan University of Technology (2022ZKCJ17).

摘　　要：The effects of Lactobacillus plantarum in microencapsulation(LPM)on intestinal development in layer chicks were investigated in this study,as well as the colonization of L.plantarum in the gut.A total of 480 healthy Hy-Line Brown layer chicks at 0 d old were randomly divided into 4 groups(8 replicates each treatment),and the diets of these birds were supplemented with nothing(control),L.plantarum(0.02 g/kg feed;10^(9) CFU/kg feed),LPM(1.0 g/kg feed;109 CFU/kg feed)and wall material of LPM(WM;0.98 g/kg feed),respectively.Compared to control,LPM improved growth performance and intestinal development of layer chicks,evidenced by significantly increased body weight,average daily gain,average daily feed intake,villus height,villus height/crypt depth,as well as weight and length of the duodenum,jejunum and ileum(P<0.05).These results could be attributed to the increased colonization of L.plantarum in the gut,which was verified by significant increases in lactic acid content,viable counts in chyme and mucosa(P<0.05),as well as a visible rise in number of strains labeled with fluorescein isothiocyanate.Meanwhile,the relative abundances of Lactobacillus and Bifidobacterium significantly increased in response to microencapsulated L.plantarum supplementation(P<0.05),accompanied by the significant up-regulation of colonization related genes(P<0.05),encoding solute carrier family,monocarboxylate transporter,activin A receptor,succinate receptor and secretogranin II.To sum up,microencapsulated L.plantarum supplementation promoted intestinal development,which could be attributed to the enhancement of L.plantarum colonization in the intestine through the mutual assistance of Bifidobacterium and interactions with colonization related transmembrane proteins.

关 键 词：Intestinal development COLONIZATION MICROBIOTA Layer chick Lactobacillus plantarum 

分 类 号：S831.5[农业科学—畜牧学]