Aflatoxin B1 decreased flesh flavor and inhibited muscle development in grass carp(Ctenopharyngodon idella)  

作　　者：Xiang Ning He Zhen Zhen Zeng Wei Dan Jiang Pei Wu Yang Liu Sheng Yao Kuang Ling Tang Shu Wei Li Lin Feng Xiao Qiu Zhou 

机构地区：[1]Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,China [2]Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China [3]Key Laboratory for Animal Disease-Resistance Nutrition,Ministry of Education,Ministry of Agriculture and Rural Affairs,Key Laboratory of Sichuan Province,Chengdu 611130,China [4]Animal Nutrition Institute,Sichuan Academy of Animal Science,Chengdu 610066,China

出　　处：《Animal Nutrition》2024年第3期27-38,共12页动物营养（英文版）

基　　金：supported by National Natural Science Foundation of China for Outst anding Youth Science Foun-dation (31922086);the Earmarked Found for China Agriculture Research System (CARS-45);National Key Research and Develop-ment Program of China (2019YFD0900200,2018YFD0900400);the Young Top Notch Talent Support Program。

摘　　要：In nature,aflatoxins,especially aflatoxin B1(AFB1),are the common mycotoxins,which cause serious health problems for humans and animals.This paper aimed to study the effects of AFB1 on flesh flavor and muscle development of grass carp(Ctenopharyngodon idella)and its mechanism.There were 1440 individual fish in total,with 6 treatments and each treatment replicated 3 times.The 6 treatments were fed a control diet with different doses of AFB1(0.04,29.48,58.66,85.94,110.43 and 146.92μg/kg diet)for 60 d.AFB1 increased myofiber diameter,as well as decreased myofiber density of grass carp muscle(P<0.05).The contents of free amino acid decreased gradually(P<0.05)as dietary AFB1 increased in the muscle of grass carp.The levels of reactive oxygen species,malonaldehyde and protein carbonyl(PC)were increased(P<0.05)with the dietary AFB1 increased.The levels of antioxidant enzyme(glutathione peroxidase,glutathione,glutathione reductase,total antioxidant capacity,anti-superoxide anion,and anti-hydroxyl radical)were decreased(P<0.05)with the dietary AFB1 increased.In addition,dietary AFB1 decreased the content of collagen,and downregulated the mRNA and protein levels of transforming growth factor-β(TGF-β)/Smads signaling pathway in grass carp muscle(P<0.05).The mRNA and protein levels of myogenic regulatory factors were downregulated in grass carp muscle(P<0.05).Furthermore,the activities of matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)were increased(P<0.05),and the protein levels of phosphorylate-38 mitogen-activated protein kinase(p-p38MAPK),phosphorylate-c-Jun N-terminal kinase,urokinase-type plasminogen activator(uPA),MMP-2 and MMP-9 were upregulated(P<0.05),but collagenⅠ,lamininβ1 and fibronectin were downregulated(P<0.05)with the dietary AFB1 increased in the muscle of grass carp.Based on the results of this study,we can draw the following conclusion:dietary AFB1 might damage flesh flavor and inhibit the muscle development through MAPK/uPA/MMP/extracellular matrix(ECM)signaling pathway in gr

关 键 词：Aflatoxin B1 Flesh flavor Muscle development Grass carp 

分 类 号：S965.112[农业科学—水产养殖]