Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot(Scophthalmus maximus)via the TOR/AAR/AMPK pathways  

作　　者：Zezheng Qi Nan Bai Qing Li Shihui Pan Min Gu 

机构地区：[1]Marine College,Shandong University,Weihai,Shandong,China [2]Key Laboratory of Modern Marine Ranching Technology of Weihai,Weihai,Shandong,China

出　　处：《Animal Nutrition》2024年第3期84-95,共12页动物营养（英文版）

基　　金：supported by Nat ional Key R&D Program of China (Grant No.2019YFD0900200);Shandong Provincial Natural Science Foundation (Grand No.ZR2022MC097 and ZR202111140103).

摘　　要：Clostridium autoethanogenum protein(CAP)is a promising protein source for aquaculture;however,how CAP influences fish quality is worth extensive research.We randomly allocated 630 turbot with initial body weights of about 180 g into 6 groups,with fishmeal-based control diet or diet with CAP replacing 15%(CAP15),30%(CAP30),45%(CAP45),60%(CAP60),or 75%(CAP75)of fishmeal protein.After a 70-d feeding trial,the fillet yield(P=0.015)and content of protein(P=0.017),collagen(P 0.05).By contrast,the muscle hardness increased linearly with increasing CAP(P=0.004),accompanied by linear reduction of muscle fiber area(P=0.003)and expression of myogenesis-related genes,including cathepsin D(ctsdP<0.001)and muscle ring finger protein 1(murf 1,P<0.001).Phosphorylation of protein kinase B(Akt,P<0.001),target of rapamycin(TOR,P=0.001),eukaryotic initiation factor 4E-binding protein 1(4E-BP1,P<0.001),and ribosomal protein S6(S6,P<0.001)decreased linearly;however,phosphorylation of AMP-activated protein kinase(AMPK,P<0.001),eukaryotic initiation factor 2α(eIF2α,P<0.001),and the abundance of activating transcription factor 4(ATF4,P<0.001)increased with increasing CAP,suggesting that the TOR signaling pathway was inhibited,and the amino acid response(AAR)and AMPK pathways were activated.Additionally,expression of genes related to protein degradation,including myogenic factor 5(myf 5,P<0.001),myogenic differentiation(myod,P<0.001),paired box 7(pax 7,P<0.001),and ctsd(P<0.001),decreased linearly with increasing CAP.In conclusion,CAP could be used to replace up to 15%of fishmeal without negatively impacting turbot quality.However,higher levels of CAP decreased fillet yield,muscle protein content,and muscle fiber diameter while increasing muscle hardness,which could be attributed to the inhibition of the TOR pathway and activation of the AAR and AMPK pathways.

关 键 词：Bacterial meal Clostridium autoethanogenum TURBOT Fish quality Muscle growth 

分 类 号：S965.399[农业科学—水产养殖]