马钱苷元对白血病细胞增殖及CDC20/MAD2表达的影响  

Effects of Loganetin on proliferation of white blood cells and CDC20/MAD2 expression

作　　者：张月明 罗雅琴[2] 张玉梅 丁继元 王敬毅[2] ZHANG Yue-ming;LUO Ya-qin;ZHANG Yu-mei;DING Ji-yuan;WANG Jing-yi(First Clinical College of Medicine,Shandong University of Traditional Chinese Medicine,Ji'nan 250000,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Ji'nan 250000,China;College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Ji'nan 250000,China)

机构地区：[1]山东中医药大学第一临床医学院,山东济南250000 [2]山东中医药大学附属医院,山东济南250000 [3]山东中医药大学中医学院,山东济南250000

出　　处：《时珍国医国药》2024年第9期2058-2061,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(82104609);山东省卫健委基金项目(202003041150);山东省自然科学基金(ZR2023LZY002)。

摘　　要：目的探讨马钱苷元对人急性髓系白血病MOLM-13细胞增殖过程中Cdc20及Mad2的影响。方法不同浓度马钱苷元处理MOLM-13细胞24、48、72 h后CCK-8法检测细胞增殖情况;流式细胞术检测不同浓度马钱苷元作用于MOLM-13细胞24h后细胞周期及凋亡的变化;Western blot检测不同浓度马钱苷元作用于MOLM-13细胞48h后Mad2和Cdc20蛋白表达情况。结果马钱苷元能显著抑制MOLM-13细胞增殖,促进MOLM-13细胞凋亡,细胞周期阻滞于G2/M期,Mad2和Cdc20蛋白表达水平均降低。结论马钱苷元对人急性髓系白血Molm细胞增殖具有较明显的抑制作用,其机制可能与调节Mad2和Cdc20的蛋白表达水平,影响细胞有丝分裂有关。Objective To investigate the effects of loganetin on Cdc20 and Mad2 during the proliferation of human acute myeloid leukemia MOLM-13.Methods The proliferation of MOLM-13 cells was detected by CCK-8 method after 24,48 and 72 h treatment with different concentrations of loganetin.Flow cytometry was used to detect the changes of cell cycle and apoptosis of MOLM-13 cells after 24h treatment with different concentrations of loganetin.Western blot analysis of Mad2 and Cdc20 protein expression in MOLM-13 cells treated with different concentrations of loganetin for 48h.Results Loganetin could significantly in-hibit the proliferation of MOLM-13 cells,Promote the apoptosis of MOLM-13 cells,arrest the cell cycle in G2/M phase,and decrease the expression levels of Mad2 and Cdc20 Protein.Conclusion Loganetin has a significant inhibitory effect on the prolif-eration of MOLM-13 cells in human acute myeloid leukemia,and the mechanism may be related to regulating the expression lev-els of Mad2 and Cdc20 protein and influencing cell mitosis.

关 键 词：马钱苷元 急性髓系白血病 Cdc20 MAD2 有丝分裂 

分 类 号：R285.5[医药卫生—中药学]