蒽贝素对MCF-7乳腺癌细胞荷瘤裸鼠肿瘤生长的影响  

Antitumor Effect of Embelin on MCF-7 Breast Cancer Xenograft in Nude Mice

作　　者：苏华 郑海平 柴玲 甘彩玉 曾宪彪 刘果 周光宇 SU Hua;ZHENG Haiping;CHAI Ling;GAN Caiyu;ZENG Xianbiao;LIU Guo;ZHOU Guangyu(Guangxi Institute of Chinese Medicine&Pharmaceutical Science,Nanning,Guangxi,China 530022;Guilin Medical University,Guilin,Guangxi,China 541001;Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi,China 530000)

机构地区：[1]广西壮族自治区中医药研究院,广西南宁530022 [2]桂林医科大学,广西桂林541001 [3]广西中医药大学附属瑞康医院,广西南宁530000

出　　处：《中国药业》2024年第20期34-38,共5页China Pharmaceuticals

基　　金：广西壮族自治区中医药管理局自筹经费科研课题[GXZYZ20210335];广西壮族自治区卫生健康委员会自筹经费科研课题[Z20211620]。

摘　　要：目的探讨蒽贝素对7乳腺癌MCF-细胞荷瘤裸鼠肿瘤生长的影响。方法取Balb/c-nu雌性裸鼠30只,随机分为模型组[等体积0.025%氨水溶液(pH 8.2)],5-氟尿嘧啶组(20 mg/kg),以及(蒽贝素)高、中、低剂量组(400,200,100μg/kg),各6只。取0.1 mL MCF-7细胞悬液皮下接种于裸鼠双侧腋下,以复制乳腺癌荷瘤裸鼠模型。建模成功后,各组裸鼠灌胃(或腹腔注射)相应药物或等体积0.025%氨水溶液(pH 8.2),均每日1次,连续14 d。末次给药后,称定瘤体质量并计算抑瘤率;苏木素-伊红(HE)染色观察肿瘤细胞形态学,Tunel染色分析肿瘤细胞凋亡情况并计算凋亡率;采用酶联免疫吸附试验(ELISA)法检测血清中肿瘤坏死因子-α(TNF-α)和白细胞介素6(IL-6)水平;免疫组化染色法检测Caspase-3蛋白表达水平。结果与模型组比较,5-氟尿嘧啶组和高剂量组裸鼠瘤体质量显著减小(P<0.01或P<0.05);各给药组细胞凋亡率显著升高;5-氟尿嘧啶组和高、中剂量组裸鼠血清TNF-α水平显著升高(P<0.01或P<0.05);各给药组Caspase-3蛋白表达水平显著升高(P<0.01)。结论蒽贝素可促进荷瘤裸鼠MCF-7细胞凋亡,其机制可能与上调Caspase-3蛋白表达有关。Objective To investigate the antitumor effect of embelin on the MCF-7 breast cancer xenograft in nude mice.Methods Thirty female Balb/c-nu nude mice were selected and randomly divided into the model group[equal volume of 0.025%ammonia solution(pH 8.2)],the 5-fluorouracil group(20 mg/kg),the embelin high-,medium-,and low-dose groups(400,200,100μg/kg),with six mice in each group.The MCF-7 cell suspension(0.1 mL)was subcutaneously inoculated into the bilateral armpits of nude mice to construct the breast cancer xenograft in nude mice.After successful modeling,the nude mice in each group were given corresponding drugs or an equal volume of 0.025%ammonia solution(pH 8.2)by gavage or intraperitoneal injection once a day for 14 d.After the last administration,the tumor mass was weighed,and the tumor inhibition rate was calculated;the hematoxylin-eosin(HE)staining was used to observe tumor cell morphology,the Tunel staining was used to analyze tumor cell apoptosis and calculate apoptosis rate;the enzyme-linked immunosorbent assay(ELISA)was used to detect the tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels in serum;the immunohistochemical staining was used to detect the expression level of Caspase-3 protein.Results Compared with those in the model group,the tumor mass in the 5-fluorouracil group and embelin high-dose group significantly decreased(P<0.01 or P<0.05);the apoptosis rate in each drug group significantly increased;the serum TNF-αlevel in the 5-fluorouracil group and embelin high-and medium-dose groups significantly increased(P<0.01 or P<0.05);the expression level of Caspase-3 protein in each drug group significantly increased(P<0.01).Conclusion Embelin can promote the apoptosis of MCF-7 cells in nude mice,and its mechanism may be related to the upregulation of Caspase-3 protein expression.

关 键 词：蒽贝素 MCF-7细胞 乳腺癌 雌性裸鼠 细胞凋亡 CASPASE-3蛋白 

分 类 号：R965[医药卫生—药理学] R285[医药卫生—药学]