麻黄汤对模型大鼠鼻黏膜免疫屏障功能的影响  

Effect of Mahuang Decoction on the Immune Barrier Function of Nasal Mucosa in Model Rats

作　　者：朱紫陌 崔白梅 杨居崩 赵显芳 李秀芳 ZHU Zimo;CUI Baimei;YANG Jubeng;ZHAO Xianfang;LI Xiufang(School of Traditional Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming,Yunnan,China 650500;Gejiu Hospital of Integrated Traditional Chinese and Western Medicine,Honghe,Yunnan,China 661000)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]云南省红河哈尼族彝族自治州个旧市中西医结合医院,云南红河661000

出　　处：《中国药业》2024年第20期38-45,共8页China Pharmaceuticals

基　　金：国家自然科学基金[81860724];云南省科技厅科技计划项目[202001AZ070001-001,202305AC160043]。

摘　　要：目的探讨麻黄汤对模型大鼠鼻黏膜屏障功能的影响及作用机制。方法以冷-热循环刺激复制鼻黏膜免疫功能低下大鼠模型,并予麻黄汤灌胃,取鼻黏膜进行转录组学测序,并用qPCR和Western blot法验证结果。结果给予模型大鼠麻黄汤后,可影响β-干扰素水平及细胞外基质、细胞因子应答等进程,并通过影响肿瘤坏死因子信号通路、JAK-STAT信号通路等发挥作用。麻黄汤通过激活IL-22/STAT3通路上调LCN2、S100A8、MUC5b、MMP13、CXCL10、CCL20、IL17RA差异基因的表达及IL-22、STAT3、p-STAT3、p-STAT1的蛋白表达,并降低TRAF6的mRNA表达。结论麻黄汤可通过IL-22/STAT3信号通路提高模型大鼠鼻腔局部免疫屏障功能。Objective To investigate the effect of Mahuang Decoction on the nasal mucosal barrier function in model rats and relevant mechanism.Methods Rat models of nasal mucosal immune dysfunction were constructed by cold-hot cycle stimulation and were given Mahuang Decoction by gavage.The nasal mucosa was taken for transcriptome sequencing,and the qPCR and Western blot were used for verification test.Results Mahuang Decoction could affect theβ-interferon level,extracellular matrix,response to cytokines and other processes in model rats,and it also played a role by affecting the tumor necrosis factor signaling pathway,JAK-STAT signaling pathway,etc.Mahuang Decoction upregulated the expression of differential genes including LCN2,S100A8,MUC5b,MMP13,CXCL10,CCL20,IL17RA and the protein expression of IL-22,STAT3,p-STAT3 and p-STAT1,and decreased the mRNA expression of TRAF6 by activating the IL-22/STAT3 pathway.Conclusion Mahuang Decoction can improve the local immune barrier function in the nasal cavity of model rats through the IL-22/STAT3 signaling pathway.

关 键 词：麻黄汤 大鼠 鼻黏膜 白细胞介素22 信号转导和转录激活因子3 免疫屏障 转录组学 

分 类 号：R965[医药卫生—药理学] R286[医药卫生—药学]