日本对虾热休克蛋白基因家族的鉴定及在发育时期的表达  

Genome-Wide Identification of the HSP Gene Family in Penaeus japonicus and Their Expression Characteristics During Development Stages

作　　者：边雪琼 任宪云[2,3] 王君霞 贾少婷 郭建军 赵旷诚 刘萍 李健[2,3] 李吉涛 BIAN Xueqiong;REN Xianyun;WANG Junxia;JIA Shaoting;GUO Jianjun;ZHAO Kuangcheng;LIU Ping;LI Jian;LI Jitao(National Experimental Teaching Demonstration Center of Aquatic Science,Shanghai Ocean University,Shanghai 201306,China;State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao Marine Science and Technology Center,Qingdao 266237,China;Rizhao Ocean and Fishery Research Institute,Rizhao 276800,China;Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]海水养殖生物育种与可持续产出全国重点实验室、中国水产科学研究院黄海水产研究所,山东青岛266071 [3]青岛海洋科技中心海洋渔业科学与食物产出过程功能实验室,山东青岛266237 [4]日照市海洋与渔业研究院,山东日照276800 [5]江苏海洋大学,江苏连云港222005

出　　处：《渔业科学进展》2024年第6期119-132,共14页Progress in Fishery Sciences

基　　金：山东省农业良种工程项目(2023LZGC020);2023年青岛市海洋科技创新专项海洋产业关键技术攻关项目(23-1-3-hygg-21-hy);中国水产科学研究院基本科研业务费(20603022022021,20603022023003);中国水产科学研究院基本科研业务费(2023TD50)共同资助;国家虾蟹产业技术体系(CARS-48)。

摘　　要：热休克蛋白(HSPs)是一类广泛存在于原核和真核生物细胞的亚细胞区室中具有高度保守性的蛋白质,具有参与蛋白质折叠、蛋白质修复和免疫等多种功能,在机体生长发育过程中发挥重要作用。为进一步研究日本对虾(Penaeus japonicus)hsp基因家族特征及在胚胎发育时期的表达特征,本研究通过生物信息学技术对日本对虾hsp进行鉴定,并对其结构域、基因结构、染色体定位、编码蛋白的理化性质和在胚胎不同发育时期的表达特征进行分析,鉴别出15个Pjhsp基因,包括1个Pjhsp10、2个Pjhsp20、2个Pjhsp40、1个Pjhsp60、6个Pjhsp70和3个Pjhsp90,其中,7个蛋白的理化性质较稳定,不稳定系数小于40;亚细胞定位结果显示,7个Pj HSP定位到细胞质,2个定位到细胞核,2个定位到细胞外基质,2个定位到内质网,2个定位到线粒体。Pjhsp基因家族中Pjhsp40-15.349、Pjhsp70-39.287和Pjhsp70-1.298等在胚胎时期大量表达;Pjhsp20、Pjhsp70-3.662、Pjhsp70-15.369、Pjhsp70-32.916和Pjhsp90-12.759等在幼体发育时期大量表达。本研究明确了日本对虾hsp家族的基因特征、系统进化关系及其在发育时期的表达规律,丰富了日本对虾hsp家族的基础信息,为进一步探讨该家族在日本对虾生长发育中的作用提供了参考。Heat shock proteins(HSPs),also known as heat stress proteins,can be divided into five main families,including HSP100,HSP90,HSP70,HSP60,and small heat shock proteins(sHSPs),according to their molecular weight.Further studies have shown that HSPs represent a class of highly conserved proteins that widely exist in the subcellular compartments of prokaryotic and eukaryotic cells.They have many functions,such as protein folding,repair,and immunity,and play important roles in organism growth and development.For example,Dmhsp70-A and Dmhsp70-B showed similar trends in the development of asexual embryos in Daphnia magna.However,they exhibited different trends in the development of sexual embryos despite high expression in both embryo types.Penaeus japonicus is one of the most important commercial shrimp species in China,Japan,and Southeast Asian countries.Relevant studies have shown that hsp genes widely exist in P.japonicus and play important roles in growth and development.For example,hsp10 and hsp60 were expressed in all tissues of P.japonicus,and the differential expression of hsp60 was identified in the early development stages.However,previous studies were based on single genes,and more systematic studies based on the role of the hsp gene family in the growth and development of P.japonicus have not been published.Our laboratory has completed the genome sequence of P.japonicus,which provides basic data for analyzing the molecular mechanism of hsp in the growth and development of P.japonicus.To further study the characteristics of the hsp gene family in P.japonicus and the expression characteristics of hsp genes during different development stages,the hsp gene family of P.japonicus was identified and analyzed using bioinformatics methods,and variation in its expression during the development of P.japonicus was measured using reverse transcription and quantitative real-time(RT-qPCR).Fifteen members of the hsp gene family were identified from the whole genome of P.japonicus via bioinformatics analysis,and their gene st

关 键 词：日本对虾 热休克蛋白 发育时期 表达规律 

分 类 号：S917[农业科学—水产科学]