桃多胺合成S-腺苷甲硫氨酸脱羧酶基因家族鉴定及对非生物胁迫的响应  

Identification of S-adenosy lmethionine Decarboxylase Gene Family Involved in Polyamines Synthesis in Peach and Its Response to Abiotic Stresses

作　　者：侯小进 宋小蝶 张梦 张晓娜 王格格 王保全 HOU Xiaojin;SONG Xiaodie;ZHANG Meng;ZHANG Xiaona;WANG Gege;WANG Baoquan(College of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang Henan 453003,China)

机构地区：[1]河南科技学院园艺园林学院,河南新乡453003

出　　处：《西南大学学报（自然科学版）》2024年第10期107-117,共11页Journal of Southwest University(Natural Science Edition)

基　　金：国家重点研发计划项目(2018YFD1000300);河南省科技攻关项目(105020224050);河南科技学院2018年博士科研启动项目(207010618005);河南科技学院2023年大学生创新创业训练计划项目(2023CX059)。

摘　　要：S-腺苷甲硫氨酸脱羧酶(SAMDC)是植物多胺合成途径中的一个关键酶,通过调控植物体内多胺的含量来影响植物对逆境胁迫的响应.为探究桃PpSAMDCs基因的功能,利用生物信息学对桃PpSAMDCs基因进行鉴定及分析其蛋白序列特征,并采用qRT-PCR分析PpSAMDCs基因在非生物胁迫下的表达模式.结果表明:在桃中共鉴定出3个PpSAMDCs基因,进化分析显示PpSAMDC1位于植物外群,PpSAMDC2和PpSAMDC3与蔷薇科植物SAMDC亲缘关系近.PpSAMDCs均为亲水性蛋白,二级结构以无规则卷曲为主,无跨膜区和信号肽.PpSAMDCs与多胺合成相关酶及甲基化转移酶发生互作.PpSAMDCs启动子上含有多个响应逆境胁迫的元件.荧光定量结果表明:PpSAMDC1受干旱和NaCl的诱导表达,但对低温无应答;PpSAMDC2参与桃对干旱、低温和盐害等逆境胁迫的调控;干旱胁迫负向调控PpSAMDC3的表达,但低温和盐胁迫却诱导该基因的表达.S-adenosylmethionine decarboxylase(SAMDC)is a key enzyme in plant polyamine synthesis pathway,which affects plant response to stress by regulating polyamines content.In order to explore the function of PpSAMDC genes in peach,PpSAMDC genes were identified and the protein sequence characteristics were analyzed using bioinformatics in the study.Real-time quantitative PCR was used to analyze expression pattern of PpSAMDCs under abiotic stresses.The results showed three PpSAMDCs were identified in the peach genome.The results of phylogenic analysis indicated that PpSAMDC1 was located in outer group,while PpSAMDC2 and PpSAMDC3 were closely relative to SAMDC proteins of Rosaceae plants.All the PpSAMDCs proteins were hydrophilic and acidic proteins.The random coil was the main secondary structure of the protein,and no transmembrane regions and signal peptide were existed in all the PpSAMDC protein sequences.PpSAMDCs interacted with polyamine synthesis related enzymes and DNA methylation transferase.The multiple cis-acting elements response to stresses were found in the PpSAMDCs promoter sequences.Quantitative PCR results showed that PpSAMDC1 expression was induced by drought and NaCl,but not affected by low temperature.PpSAMDC2 was simultaneously involved in the regulation of peach in response to the stresses such as drought,low temperature and salt stress.Expression of PpSAMDC3 was negatively regulated by drought stress,but induced by low temperature and salt stresses.The results of this study lay a foundation for further research on biological function and molecular mechanism of stress resistance of PpSAMDCs genes in peach.

关 键 词：桃 PpSAMDCs基因 克隆 生物信息学分析 表达分析 

分 类 号：S662.1[农业科学—果树学]