水稻CHYR家族全基因组鉴定与表达分析  

Genome-wide Identification and Expression Analysis of CHYR Gene Family in Rice

作　　者：王研 才晓溪 沈阳 金军 董伟峰 孙明哲[1] 贾博为 孙晓丽[1] Wang Yan;Cai Xiaoxi;Shen Yang;Jin Jun;Dong Weifeng;Sun Mingzhe;Jia Bowei;Sun Xiaoli(Crop Stress Molecular Biology Laboratory,Heilongjiang Bayi Agricultural University,Daqing,163319)

机构地区：[1]黑龙江八一农垦大学,作物逆境分子生物学实验室,大庆163319

出　　处：《分子植物育种》2024年第20期6624-6632,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31971826,U20A2025,32101672);中央支持地方高校改革发展资金人才培养支持计划项目(22B014);黑龙江八一农垦大学研究生创新科研项目(YJSCX2021-Y42)共同资助。

摘　　要：CHYR家族是一类含有CHY zinc finger和RING finger结构域的基因,广泛参与植物的耐逆应答过程。本研究通过生物信息学手段,在全基因组水平上筛选鉴定出7个水稻CHYR基因。通过系统发生分析,将水稻CHYR家族分为3个亚家族,且各组内的基因在基因结构和保守基序上比较保守;染色体定位分析表明水稻CHYR基因分布在5条染色体上,进一步不同物种共线性分析表明水稻和玉米的基因同源性较高;组织和胁迫表达模式分析表明,CHYR基因在水稻各组织均有较高的表达,且参与盐、干旱和低温胁迫应答;蛋白互作分析发现,OsCHYR可能通过与转录因子、泛素结合酶、泛素连接酶互作发挥功能。CHYR family,which is a group of genes containing CHY zinc finger domain and RING finger domain,are widely involved in plants response to adverse conditions.In this study,a total of 7 rice CHYR genes were identified by using bioinformatics.Phylogenetic analysis depicted that the 7 rice CHYR genes could be further divided into 3 subfamilies.Furthermore,genes in different groups were conserved in term of gene structure and conserved motif.Chromosome location analysis showed that 7 OsCHYR genes were distributed on 5 different chromosomes.Further syntenic analysis revealed that rice and maize showed higher gene homology.Rice tissue expression data analysis indicated that OsCHYR genes were highly expressed in all rice detected tissues,and induced by salt,drought and cold stress.Protein-protein interaction analysis showed that OsCHYR may function by interacting with transcription factors,ubiquitin building enzyme and ubiquitin ligase enzyme.

关 键 词：水稻 CHYR基因家族 全基因组鉴定 表达分析 

分 类 号：S511[农业科学—作物学]