内异痛经灵调控Wnt7a/β-catenin信号通路改善子宫内膜异位症的实验研究  

Experimental study on the effect of Neiyi Tongjing Ling in the treatment of endometriosis by regulating the Wnt7a/β-catenin signaling pathway

作　　者：钟璐[1,2] 申梦琦[1] 蓝幼梅 苏圣琪 黄群 方刚 庞宇舟[1] ZHONG Lu;SHEN Mengqi;LAN Youmei;SU Shengqi;HUANG Qun;FANG Gang;PANG Yuzhou(Guangxi University of Chinese Medicine,Nanning 530001,China;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China)

机构地区：[1]广西中医药大学,南宁530001 [2]广西中医药大学附属瑞康医院,南宁530011

出　　处：《吉林中医药》2024年第10期1221-1226,共6页Jilin Journal of Chinese Medicine

基　　金：2020年度广西高校中青年教师科研基础能力提升项目(2021KY0299);2022年中国民族医药学会科研项目(2022M1042-310103);2022年中医药事业传承与发展结算补助资金-中医临床优秀人才研修项目(桂财社函[2022]136号)。

摘　　要：目的旨在明确内异痛经灵(NYTJL)对大鼠子宫内膜异位症的改善效果并从调控Wnt7a/β-catenin信号通路明确其分子机制。方法将30只雌性,SPF级,SD大鼠,随机分为5组,每组6只。除Control组外,Sham组、Model组、高剂量内异痛经灵(NYTJL-H,40 mg/kg)组和低剂量内异痛经灵(NYTJL-L,20 mg/kg)组均将大鼠子宫内膜固定在腹壁进行上造模;并对应灌胃给药,干预结束后,利用HE染色观察子宫内膜组织形态变化,应用Ki67免疫组化及TUNEL染色检测子宫内膜组织中细胞增殖及凋亡,应用RT-qPCR及蛋白印迹检测子宫内膜组织中Wnt7a及β-catenin的表达,以免疫荧光检测β-catenin的表达。结果与Model组比较,NYTJL高剂量组可以显著改善子宫内膜的组织学形态,降低浸润细胞数量,促进细胞分散排布,减少腺体数量,缩小腺腔体积;与Model组比较,NYTJL-H组和NYTJL-L组均可显著促进异位子宫内膜细胞凋亡,抑制异位子宫内膜细胞增殖;与Control和Sham组比较,Model组Wnt7a和β-catenin的mRNA和蛋白表达均显著上升,而与Model组比较,NYTJL-H组和NYTJL-L组对Wnt7a和β-catenin的表达有显著的抑制作用。结论NYTJL可以抑制Wnt7a/β-catenin信号通路进而促进异位子宫内膜细胞凋亡,进而治疗子宫内膜异位症。Objective To investigate the effect of Neiyi Tongjing Ling(NYTJL)in the treatment of endometriosis in rats and its molecular mechanism of regulating the Wnt7a/β-catenin signaling pathway.Methods Thirty female SPF SD rats were randomly divided into five groups,with six rats in each.Except for the control group,a model was established in those rats in the sham group,the model group,the high-dose Neiyi Tongjing Ling(NYTJL-H,40 mg/kg)group,and the low-dose Neiyi Tongjing Ling(NYTJL-L,20 mg/kg)group,and corresponding drugs were orally administered in each group.After the intervention,HE staining was used to observe the morphological changes of endometrial tissue.Ki67 immunohistochemistry and TUNEL staining were used to detect the cell proliferation and apoptosis in endometrial tissue.RT-qRT,RT-qPCR and Western blot were used to detect the expressions of Wnt7a andβ-catenin in endometrial tissue,and immunofluorescence was used to detect the expression ofβ-catenin.Results Compared with the model group,the high-dose NYTJL group significantly improved the histological morphology of endometrium,reduced the number of infiltrating cells,promoted cell dispersion,reduced the number of glands,and reduced the volume of glandular cavities.Compared with the model group,both the NYTJL-H group and the NYTJL-L group significantly promoted the apoptosis of ectopic endometrial cells and inhibited their proliferation.Compared with the control and sham groups,the mRNA and protein expressions of Wnt7a andβ-catenin in the model group were significantly increased,while compared with the model group,the NYTJL-H and NYTJL-L groups had a significant inhibitory effect on the expressions of Wnt7a andβ-catenin.Conclusion NYTJL can inhibit the Wnt7a/β-catenin signaling pathway and promote the apoptosis of ectopic endometrial cells,and thus treat endometriosis.

关 键 词：内异痛经灵 子宫内膜异位症 Wnt7b Β-CATENIN 细胞增殖 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]