三种布鲁氏菌病抗体检测方法的比较  

Comparison of Three Detection Methods for Antibodies Against Brucellosis

作　　者：参都哈西·加吾丁 陆桂丽 陈古丽 哈力旦木 薛晶 韩涛 王陆宝 古丽扎提 伊力哈木 艾沙江 王杰 SANDUHAXI·Jiawuding;LU Guili;CHEN Guli;HALIDANMU;XUE Jing;HAN Tao;WANG Lubao;GULIZHATI;YILIHAMU;AISHAJIANG;WANG Jie(Institute of Veterinary Medicine/Research Center of Animal Clinical Medicine,Xinjiang Academy of Animal Science,Urumqi 830013,China;Urumqi Customs,Urumqi 830011,China)

机构地区：[1]新疆畜牧科学院兽医研究所/新疆畜牧科学院动物临床医学研究中心,乌鲁木齐830013 [2]乌鲁木齐海关,乌鲁木齐8300110

出　　处：《草食家畜》2024年第5期40-43,共4页Grass-Feeding Livestock

基　　金：新疆维吾尔自治区农业农村厅项目“奶产业技术体系”(XJARS-11)。

摘　　要：【目的】旨在对酶联免疫吸附试验(i-ELISA)、竞争酶联免疫吸附试验(c-ELISA)和胶体金免疫层析法(GICA)用于检测布病抗体的敏感性、特异性和符合率进行比较。【方法】对实验室保存的40份牛血清样品,以布鲁氏菌病虎红平板凝集试验(RBT)、c-ELISA和国标推荐的布鲁氏菌病试管凝集试验(SAT)方法作为标准,确定布病的阳性和阴性样品。根据已确定的布病样品和40份样品的检测结果,对i-ELISA、c-ELISA和GICA与SAT的敏感性、特异性和符合率进行比较。【结果】对已确定的布病样品检测结果显示,c-ELISA与SAT的特异性和符合率均为100%;GICA 3的特异性和符合率最低,分别为91.4%和92.1%;i-ELISA、GICA 1、GICA 2和GICA 4的特异性和符合率均相同,分别为94.1%和94.7%。通过对40份血清样品的检测结果比较分析可知,RBT和4种GICA的敏感性较低,在66.7%~75%之间。【结论】c-ELISA的敏感性、特异性和符合率均为100%;4种GICA的敏感性较低,存在一定比列的假阳性和假阴性样品。【Objective】In order to compare the sensitivity,specificity and concordance rate of three methods consisting of i-ELISA、c-ELISA and GICA in detecting brucellosis antibodies.【Methods】For 40 bovine serum samples kept in the laboratory,the positive and negative samples of brucellosis were determined by RBT,c-ELISA as well as SAT method recommended by the national standard.Then the sensitivity,specificity and coincidence rate of i-ELISA,c-ELISA and GICA were compared by detecting Brucella-positive serum samples and the 40 clinical samples.【Results】 For the Brucella-positive serum samples,compared with the results of SAT,the specificity and coincidence rate of c-ELISA were 100%,those of GICA 3 were the lowest,91.4% and 92.1% respectively.The specificity and coincidence rate of i-ELISA,GICA 1,GICA 2 and GICA 4 were all the same,which were 94.1% and 94.7% respectively.For the 40 clinical serum samples,the sensitivity of RBT and 4 kinds of GICA was lower,ranging from 66.7% to 75%.【Conclusion】The sensitivity,specificity and coincidence rate of c-ELISA were all 100%,while the sensitivity of the 4 kinds of GICA was lower,and there were a certain proportion of false positive and negative samples.

关 键 词：布鲁氏菌病 检测 敏感性 特异性 符合率 

分 类 号：S858.23[农业科学—临床兽医学]