鸭源A型鹦鹉热衣原体的分离鉴定  

Isolation and identification of genotype A Chlamydia psittaci strain from duck

作　　者：李兆才[1] 刘萍[1] 李赟辉 张淮瑜 肖乾 刘春国 周继章[1,4] LI Zhao-cai;LIU Ping;LI Yun-hui;ZHANG Huai-yu;XIAO Qian;LIU Chun-guo;ZHOU Ji-zhang(State Key Laboratory for Animal Disease Control and Prevention,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;College of Veterinary Medicine Northwest A&F University,Yangling 712100,China;Group Testing Center,Shandong Sinder Technology Co.,Ltd.,Qingdao 266100,China;College of Veterinary Medicine,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]中国农业科学院兰州兽医研究所,动物疫病防控全国重点实验室,兰州730046 [2]西北农林科技大学动物医学院,杨凌712100 [3]山东信得科技股份有限公司集团检测中心,青岛266100 [4]兰州大学动物医学与生物安全学院,兰州730000

出　　处：《中国人兽共患病学报》2024年第9期823-828,共6页Chinese Journal of Zoonoses

基　　金：国家重点研发计划项目(No.2022YFC2304001);中国农业科学院科技创新工程项目(No.CAAS-ASTIP-2021-LVRI)。

摘　　要：目的对疑似鹦鹉热衣原体感染鸭进行检测、病原分离培养和基因型鉴定。方法从山东某鸭场采集病鸭肺脏,进行衣原体荧光PCR检测;将阳性样本匀浆和无菌处理后,接种L929细胞,连续传代培养,通过Giemsa染色和电子显微镜观察细胞内衣原体包涵体的形成;提取分离株DNA并测序,分析16S rRNA、16-23S IGS和ompA基因序列进行种属鉴定和基因分型。结果成功分离到1株鸭源鹦鹉热衣原体,基因型鉴定为A型。结论鸭源A型鹦鹉热衣原体的分离鉴定扩大了对A型菌株感染宿主范围的认识,为其生物学特性、致病性和防控技术及公共卫生等相关研究提供基础资料。Chlamydia psittaci is a worldwide distributed zoonotic pathogen that infects a variety of birds.In order to characterization of the duck originated C.psittaci strains,lung samples were collected from suspected infection ducks and was detected by real-time PCR.The positive samples were homogenized in phosphate buffered saline with kanamycin and streptomycin,and then inoculated onto L929 cells monolayer.After several sets of passages,chlamydial inclusions of the isolate in cultured cells were observed after Giemsa staining or by electron microscope.For species identification,16S rRNA,16-23S IGS gene fragments were sequenced and analyzed.Genotyping of the isolate was performed by comparative analysis of the obtained ompA gene sequence with that of different genotype of C.psittaci strains.A C.psittaci strain was successfully isolated from the lung sample of duck by cell culture and was identified as genotype A.This study expanded our understanding of the host range of genotype A C.psittaci strain,and provided basis for further research on the pathogenicity,transmission,and public health risk of this pathogen.

关 键 词：鹦鹉热衣原体 细胞培养 基因分型 鸭 

分 类 号：R374.2[医药卫生—病原生物学] S855.1[医药卫生—基础医学]