2022-2023年江苏省H3N2流感病毒分子特征及耐药性分析  

Molecular characteristics and drug resistance analysis of H3N2 influenza virus in Jiangsu Province from 2022 to 2023

作　　者：邓斐[1] 王慎骄[1] 余慧燕[1] 祁贤[1] 朱立国[1] 戴启刚[1] DENG Fei;WANG Shen-jiao;YU Hui-yan;QI Xian;ZHU Li-guo;DAI Qi-gang(Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China)

机构地区：[1]江苏省疾病预防控制中心,南京210009

出　　处：《中国人兽共患病学报》2024年第9期848-854,共7页Chinese Journal of Zoonoses

基　　金：江苏省卫生健康委员会科研项目(No.M2020026)。

摘　　要：目的根据2022-2023年江苏省监测年度流感病毒实验室检测结果,对H3N2流感病毒的基因特征和耐药性进行深度分析,评估疫苗和药物的有效性。方法对全省流感网络实验室分离的29株H3N2分离株进行全基因组扩增与测序,构建HA和NA基因进化树。分析抗原表位、糖基化位点以及耐药位点的突变特性,并采用荧光发光法对流感病毒神经氨酸酶进行检测。结果江苏省29株H3N2分离株的同源性较高,与国内参考株和2021-2022年度疫苗株位于同一进化分支,与国外参考株和2022-2023年度疫苗株则处于不同进化分支。与2021-2022年度疫苗株A/Cambodia/e0826360/2020相比,江苏省2022-2023年度分离的H3N2流感病毒HA蛋白发生了4个氨基酸位点的替换,NA蛋白发生了2个氨基酸位点的替换,糖基化位点、受体结合位点以及氨基酸残基保守区域未发生改变。IC_(50)结果表明,29株H3N2分离株对奥司他韦和扎那米韦两种抗病毒药物均未产生耐药。结论江苏省2022-2023年监测年度的流感病毒主要为甲型H3N2流感病毒。通过对流感病毒的HA和NA基因变异位点以及耐药性监测,及时了解病毒基因的变异情况,为选择有效的疫苗株提供科学依据,从而有助于预防和控制流感病毒的暴发和流行。On the basis of the laboratory detection results of influenza viruses in Jiangsu Province from 2022 to 2023,this study was aimed at conducting a analysis of the genetic characteristics and resistance of the H3N2 influenza virus,and evaluating the effectiveness of vaccines and drugs.The full genome of 29 H3N2 isolates submitted by provincewide influenza surveillance network laboratories was amplified and sequenced.Phylogenetic trees of the HA and NA genes were constructed.The mutation characteristics of antigenic sites,glycosylation sites,and resistance sites were analyzed,and the influenza virus neuraminidase was detected with a fluorescence luminescence method.The homology of the 29 H3N2 isolates in Jiangsu Province was relatively high.The isolates belonged to the same evolutionary branch as domestic reference strains and the 2021-2022 vaccine strains,but were in different evolutionary branches from foreign reference strains and the 2022-2023 vaccine strains.Compared with the 2021-2022 vaccine strain A/Cambodia/e0826360/2020,the H3N2 influenza viruses isolated in Jiangsu Province during 2022-2023 had four amino acid substitutions in the HA protein and two amino acid substitutions in the NA protein.No changes in glycosylation sites,receptor-binding sites,and conserved amino acid residue regions were observed.IC_(50) analysis indicated that the 29 H3N2 isolates were not resistant to the antiviral drugs oseltamivir and zanamivir.The predominant influenza viruses detected in Jiangsu Province during 2022-2023 were type A H3N2 influenza viruses.Monitoring of variations in HA and NA gene sites and influenza virus resistance aids in rapid understanding gene mutations,and can inform the selection of effective vaccine strains,and contribute to the prevention and control of influenza virus outbreaks.

关 键 词：H3N2流感病毒 疫苗 药物 基因特征 进化分析 

分 类 号：R373.1[医药卫生—病原生物学]