Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities  

作　　者：Sai Wan Mingchi Lai Xinyu Gao Mingxin Zhou Song Yang Qiang Li Fuli Li Lin Xia Yang Tan 

机构地区：[1]Qingdao C1 Refinery Engineering Research Center,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao,266101,Shandong,China [2]CAS Key Laboratory of Quantitative Engineering Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Science,Shenzhen,518055,China [3]Shenzhen Powered Carbon Biotechnology Co.,Ltd,Shenzhen,518055,China [4]School of Life Sciences,Qingdao Agricultural University,Qingdao,266109,Shandong,China [5]School of Biological Science and Technology,University of Jinan,Jinan,250022,Shandong,China

出　　处：《Synthetic and Systems Biotechnology》2024年第1期19-25,共7页合成和系统生物技术（英文）

基　　金：supported by the Shenzhen Science and Technology Program(Grant No.JCYJ20210324101014036).

摘　　要：Excessive mining and utilization fossil fuels has led to drastic environmental consequences,which will contribute to global warming and cause further climate change with severe consequences for the human population.The magnitude of these challenges requires several approaches to develop sustainable alternatives for chemicals and fuels production.In this context,biological processes,mainly microbial fermentation,have gained particular interest.For example,autotrophic gas-fermenting acetogenic bacteria are capable of converting CO,CO_(2) and H_(2) into biomass and multiple metabolites through Wood-Ljungdahl pathway,which can be exploited for large-scale fermentation processes to sustainably produce bulk biochemicals and biofuels(e.g.acetate and ethanol)from syngas.Clostridium autoethanogenum is one representative of these chemoautotrophic bacteria and considered as the model for the gas fermentation.Recently,the development of synthetic biology toolbox for this strain has enabled us to study and genetically improve their metabolic capability in gas fermentation.In this review,we will summarize the recent progress involved in the understanding of physiological mechanism and strain engineering for C.autoethanogenum,and provide our perspectives on the future development about the basic biology and engineering biology of this strain.

关 键 词：Genetic engineering Clostridium autoethanogenum Gas fermentation Carbon fixation BIOFUEL 

分 类 号：Q93[生物学—微生物学]