Engineering Rubisco to enhance CO_(2) utilization  

作　　者：Lei Zhao Zhen Cai Yin Li Yanping Zhang 

机构地区：[1]CAS Key Laboratory of Microbial Physiological and Metabolic Engineering,State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing,100101,China [2]University of Chinese Academy of Sciences,Beijing,100049,China [3]Seeds Research,Syngenta Biotechnology China,Zhong Guan Cun Life Science Park,Beijing,China

出　　处：《Synthetic and Systems Biotechnology》2024年第1期55-68,共14页合成和系统生物技术（英文）

基　　金：supported by the National Key R&D Program of China(2021YFC2103500);the National Natural Science Foundation of China(32270057);the DNL Cooperation Fund,CAS(DNL202014).

摘　　要：Ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)is a pivotal enzyme that mediates the fixation of CO_(2).As the most abundant protein on earth,Rubisco has a significant impact on global carbon,water,and nitrogen cycles.However,the significantly low carboxylation activity and competing oxygenase activity of Rubisco greatly impede high carbon fixation efficiency.This review first summarizes the current efforts in directly or indirectly modifying plant Rubisco,which has been challenging due to its high conservation and limitations in chloroplast transformation techniques.However,recent advancements in understanding Rubisco biogenesis with the assistance of chaperones have enabled successful heterologous expression of all Rubisco forms,including plant Rubisco,in microorganisms.This breakthrough facilitates the acquisition and evaluation of modified proteins,streamlining the measurement of their activity.Moreover,the establishment of a screening system in E.coli opens up possibilities for obtaining high-performance mutant enzymes through directed evolution.Finally,this review emphasizes the utilization of Rubisco in microorganisms,not only expanding their carbon-fixing capabilities but also holding significant potential for enhancing biotransformation processes.

关 键 词：RUBISCO Plant CO_(2)fixation MICROBIAL Carbon fixation pathway Synthetic biology Enzyme engineering 

分 类 号：Q94[生物学—植物学]