Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum  

作　　者：Chunjian Li Pengdong Sun Guoqing Wei Yuqi Zhu Jingyuan Li Yanfeng Liu Jian Chen Yang Deng 

机构地区：[1]College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China [2]Key Laboratory of Special Food Processing(Co-construction by Ministry and Province),Ministry of Agriculture Rural Affairs,Qingdao Agricultural University,Qingdao 266109,China [3]Shandong Technology Innovation Center of Special Food,Qingdao 266109,China [4]Qingdao Special Food Research Institute,Qingdao 266109,China [5]Qingdao Nuoan Baite Biotechnology Co.,Ltd.,Qingdao 266109,China [6]Science Center for Future Foods,Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology,and Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology,Jiangnan University,Wuxi 214122,China [7]Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China

出　　处：《Synthetic and Systems Biotechnology》2024年第1期99-107,共9页合成和系统生物技术（英文）

基　　金：funded by National Natural Science Foundation of China(no.32272279);the Key R&D project of Qingdao Science and Technology Plan(22-3-3-hygg-29-hy).

摘　　要：Creatine is a naturally occurring derivative of an amino acid commonly utilized in functional foods and pharmaceuticals.Nevertheless,the current industrial synthesis of creatine relies on chemical processes,which may hinder its utilization in certain applications.Therefore,a biological approach was devised that employs whole-cell biocatalysis in the bacterium Corynebacterium glutamicum,which is considered safe for use in food production,to produce safe-for-consumption creatine.The objective of this study was to identify a guanidinoacetate N-methyltransferase(GAMT)with superior catalytic activity for creatine production.Through employing whole-cell biocatalysis,a gamt gene from Mus caroli(Mcgamt)was cloned and expressed in C.glutamicum ATCC 13032,resulting in a creatine titer of 3.37 g/L.Additionally,the study employed a promoter screening strategy that utilized nine native strong promoters in C.glutamicum to enhance the expression level of GAMT.The highest titer was achieved using the P1676 promoter,reaching 4.14 g/L.The conditions of whole-cell biocatalysis were further optimized,resulting in a creatine titer of 5.42 g/L.This is the first report of successful secretory creatine expression in C.glutamicum,which provides a safer and eco-friendly approach for the industrial production of creatine.

关 键 词：CREATINE Corynebacterium glutamicum Whole-cell biocatalysis Guanidinoacetate N-Methyltransferase Food additive 

分 类 号：O62[理学—有机化学]