Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis  

作　　者：Huijie Sun Huashan Bai Yonghong Hu Sheng He Ruihang Wei Duotao Meng Qiong Jiang Hongping Pan Peihong Shen Qian Ou Chengjian Jiang 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Guangxi Research Center for Microbial and Enzyme Engineering Technology,College of Life Science and Technology,Guangxi University,Nanning,530004,China [2]Guangxi Key Laboratory for Green Processing of Sugar Resources,College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou,545006,China [3]Guangxi Key Laboratory of Birth Defects Research and Prevention,Guangxi Key Laboratory of Reproductive Health and Birth Defect Prevention,Guangxi Zhuang Autonomous Region Women and Children Health Care Hospital,Nanning,530033,China [4]College of Food Science and Light Industry,Nanjing Tech University,No.30,South Puzhu Road,Nanjing,211816,China

出　　处：《Synthetic and Systems Biotechnology》2024年第1期115-126,共12页合成和系统生物技术（英文）

基　　金：supported by the Funding Project of Chinese Central Government Guiding to the Guangxi Local Science and Technology Development(Grant No.GUIKEZY21195021);the Natural Science Fund for Distinguished Young Scholars of Guangxi Zhuang Autonomous Region of China(Grant No.2019GXNSFFA245011);the Funding Project of Chinese Central Government Guiding to the Nanning Local Science and Technology Development(Grant No.20231012);the Funding Projects of Guangxi Key Research and Development Plan(GUIKE AB23075173);the Funding Project of Technological Development from Angel Yeast(Chongzuo)Co.,Ltd.(Grant No.JS1006020230722019).

摘　　要：Dopamine can be used to treat depression,myocardial infarction,and other diseases.However,few reports are available on the de novo microbial synthesis of dopamine from low-cost substrate.In this study,integrated omics technology was used to explore the dopamine metabolism of a novel marine multi-stress-tolerant aromatic yeast Meyerozyma guilliermondii GXDK6.GXDK6 was found to have the ability to biosynthesize dopamine when using glucose as the substrate.14 key genes for the biosynthesis of dopamine were identified by whole genome-wide analysis.Transcriptomic and proteomic data showed that the expression levels of gene AAT2 encoding aspartate aminotransferase(regulating dopamine anabolism)were upregulated,while gene AO-I encoding copper amine oxidase(involved in dopamine catabolism)were downregulated under 10%NaCl stress compared with non-NaCl stress,thereby contributing to biosynthesis of dopamine.Further,the amount of dopamine under 10%NaCl stress was 2.51-fold higher than that of zero NaCl,which was consistent with the multi-omics results.Real-time fluorescence quantitative PCR(RT-qPCR)and high-performance liquid chromatography(HPLC)results confirmed the metabolic model of dopamine.Furthermore,by overexpressing AAT2,AST enzyme activity was increased by 24.89%,the expression of genes related to dopamine metabolism was enhanced,and dopamine production was increased by 56.36%in recombinant GXDK6AAT2.In conclusion,Meyerozyma guilliermondii GXDK6 could utilize low-cost carbon source to synthesize dopamine,and NaCl stress promoted the biosynthesis of dopamine.

关 键 词：Dopamine biosynthesis Multi-omics analysis NaCl stress Meyerozyma guilliermondii 

分 类 号：Q42[生物学—神经生物学]