白芍总苷对H_(2)O_(2)诱导下黑色素细胞的保护及作用机制  

Protective effect of total glucosides of paeony on melanocytes induced by H_(2)O_(2)and its mechanism

作　　者：杨林芳 赵肖灵 江世宾 张明[3] 程芳[1] YANG Linfang;ZHAO Xiaoling;JIANG Shibin;ZHANG Ming;CHENG Fang(Department of Dermatology,Xingtai People′s Hospital,Xingtai 054001,HeBei Province,China;Cancer Laboratory,Xingtai People′s Hospital,Xingtai 054001,HeBei Provinc,China;Dermatology Department,Beijing Youan Hospital,Capital Medical University Beijing Institute of Hepatology,Beijing 100069,China)

机构地区：[1]邢台市人民医院皮肤科,河北邢台054001 [2]邢台市人民医院肿瘤实验室,河北邢台054001 [3]首都医科大学附属北京佑安医院皮肤科,北京100069

出　　处：《天津师范大学学报（自然科学版）》2024年第5期30-35,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：邢台市科技局基金资助项目(2020zc233);河北省青年拔尖人才支持项目。

摘　　要：为了探究白芍总苷治疗白癜风的潜在作用机理,对人正常黑色素细胞PIG1进行体外培养,用不同浓度的白芍总苷对细胞进行预处理并施加H_(2)O_(2)刺激,用CCK-8法检测细胞活力,用流式细胞术检测细胞凋亡和活性氧的产生,用蛋白免疫印迹法检测细胞色素c、cleaved caspase-3、cleaved caspase-9含量以及MAPK家族成员p38及其磷酸化的表达.结果显示:①白芍总苷可有效抑制H_(2)O_(2)诱导的PIG1细胞毒性,且呈剂量依赖性;②白芍总苷减少了H_(2)O_(2)诱导的细胞活性氧的产生和细胞色素c的释放,下调了cleaved caspase-3和cleaved caspase-9的激活;③白芍总苷有效阻碍了H_(2)O_(2)诱导的p38 MAPK磷酸化.上述结果表明,白芍总苷可作为一种有效的抗氧化剂,保护黑色素细胞免受H_(2)O_(2)诱导的氧化应激损伤.To explore the potential mechanism of total glucosides of paeony(TGP)in the treatment of vitiligo,human melanocytes PIG1 cells were cultured in vitro,which were pretreated with different concentrations of total glucosides of paeony and stimulat-ed by H_(2)O_(2).CCK-8 assay was adopted to detect cell viability,flow cytometry was used to detect cell apoptosis and ROS produc-tion,and Western blotting was used to detect the expression of cytochrome c,cleaved caspase-3,cleaved caspase-9 protein,as well as MAPK family member p38 and its phosphorylation.The results were as follows:①TGP can effectively ameliorate the H_(2)O_(2)induced oxidative stress damage in PIG1 cells in a dose-dependent manner;②TGP reduced cell ROS production and the release of cytochrome c,and downregulated activation of cleaved caspase-3 and cleaved caspase-9;③The pretreatment with TGP hindered H_(2)O_(2)induced p38 MAPK phosphorylation in a time-dependent manner.The above results demonstrate that TGP can be used as an effective antioxidant to protect melanocytes from H_(2)O_(2)induced oxidative stress injury.

关 键 词：白芍总苷 白癜风 活性氧 抗氧化剂 黑色素细胞 作用机制 

分 类 号：R285[医药卫生—中药学]